Resistance to amitraz in the parasitic honey bee mite Varroa destructor is associated with mutations in the β−adrenergic-like octopamine receptor

2021

AbstractVarroa destructor is considered a major reason for high loss rate of Western honey bee (Apis mellifera) colonies. To prevent colony losses caused by V. destructor it is necessary to actively manage the mite population. Beekeepers, particularly commercial beekeepers, have few alternative treatments other than synthetic acaricides to control the parasite, resulting in intensive treatment regimens that led to the evolution of resistance in mite populations.To investigate the mechanism of the resistance to amitraz detected in V. destructor mites from French and U.S. apiaries, we identified and characterized octopamine and tyramine receptors (the known targets of amitraz) in this species…

Nitric Oxide-Releasing Drug Glyceryl Trinitrate Targets JAK2/STAT3 Signaling, Migration and Invasion of Triple-Negative Breast Cancer Cells

2021

Triple-negative breast cancer (TNBC) is a highly aggressive disease with invasive and metastasizing properties associated with a poor prognosis. The STAT3 signaling pathway has shown a pivotal role in cancer cell migration, invasion, metastasis and drug resistance of TNBC cells. IL-6 is a main upstream activator of the JAK2/STAT3 pathway. In the present study we examined the impact of the NO-donor glyceryl trinitrate (GTN) on the activation of the JAK2/STAT3 signaling pathway and subsequent migration, invasion and metastasis ability of TNBC cells through in vitro and in vivo experiments. We used a subtoxic dose of carboplatin and/or recombinant IL-6 to activate the JAK2/STAT3 signaling path…

false

2021

Purpose: Physical activity promotes a positive physical self-image, but less is known about whether the positive effect covers the general self-evaluation of adolescent appearance. This study aimed to further explore the associations between physical activity and body-esteem in Finnish adolescents, via a country-representative cross-sectional study design. Methods: The data for the study were drawn from the Finnish component of the Health Behaviour in School-aged Children (HBSC) study (2014), using surveys from 15-year-old adolescents (n=1956, mean age 15.8 years) from 122 schools. Self-evaluations of body or appearance were measured by the Body-Esteem Scale for Adolescents and Adults (BESA…

PenRed: An extensible and parallel Monte-Carlo framework for radiation transport based on PENELOPE

2021

Monte Carlo methods provide detailed and accurate results for radiation transport simulations. Unfortunately, the high computational cost of these methods limits its usage in real-time applications. Moreover, existing computer codes do not provide a methodology for adapting these kinds of simulations to specific problems without advanced knowledge of the corresponding code system, and this restricts their applicability. To help solve these current limitations, we present PenRed, a general-purpose, stand-alone, extensible and modular framework code based on PENELOPE for parallel Monte Carlo simulations of electron-photon transport through matter. It has been implemented in C++ programming la…

Revalorization of Almond By-Products for the Design of Novel Functional Foods: An Updated Review

2021

The search for waste minimization and the valorization of by-products are key to good management and improved sustainability in the food industry. The great production of almonds, based on their high nutritional value as food, especially almond kernels, generates tons of waste yearly. The remaining parts (skin, shell, hulls, etc.) are still little explored, even though they have been used as fuel by burning or as livestock feed. The interest in these by-products has been increasing, as they possess beneficial properties, caused by the presence of different bioactive compounds, and can be used as promising sources of new ingredients for the food, cosmetic and pharmaceutical industry. Additio…

Delivery of Health Care by Spanish Dental Hygienists in Private and Public Dental Services during the COVID-19 De-Escalation Phase (June 2020): A Cro…

2021

Background: The first wave of the COVID-19 pandemic in Spain posed a major challenge for Spanish dental professionals. The objective of this work is to describe the dental hygienists’ work status and employment patterns during the de-escalation phase in order to analyse the standards of knowledge, compliance with official recommendations, and dental activities both in the public health service and in the private sector. Material and Methods: A cross-sectional questionnaire was answered by Spanish dental hygienists via WhatsApp, Facebook, and Instagram. The questionnaire was piloted before it was distributed and carried out during June 2020. Results: Here, 517 dental hygienists were surveyed…

Building a Functionalizable, Potent Chemiluminescent Agent: A Rational Design Study on 6,8-Substituted Luminol Derivatives.

2021

Luminol is a prominent chemiluminescent (CL) agent, finding applications across numerous fields, including forensics, immunoassays, and imaging. Different substitution patterns on the aromatic ring can enhance or decrease its CL efficiency. We herein report a systematic study on the synthesis and photophysics of all possible 6,8-disubstituted luminol derivatives bearing H, Ph, and/or Me substituents. Their CL responses are monitored at three pH values (8, 10, and 12), thus revealing the architecture with the optimum CL efficiency. The most efficient pattern is used for the synthesis of a strongly CL luminol derivative, bearing a functional group for further, straightforward derivatization. …

Refining Prognosis in Chemoembolization for Hepatocellular Carcinoma: Immunonutrition and Liver Function

2021

A combination of albumin-bilirubin (ALBI) grading and the Prognostic Nutritional Index (PNI) was identified recently as a highly predictive tool for patients with hepatocellular carcinoma (HCC) undergoing tumor ablation. The present study evaluated this combination in patients undergoing transarterial chemoembolization (TACE). Between 2010 and 2020, 280 treatment-naïve patients were retrospectively identified. The influence of ALBI grade, PNI and the novel ALBI-PNI on the median overall survival (OS) was assessed. In the next step, the prognostic ability of the combined approach was compared to established scoring systems. Both ALBI grade 2−3 and a low PNI were highly predictive for median …

false

2021

The domestication and spreading of grapevine as well as the gene flow history had been described in many studies. We used a high-quality 7k SNP dataset of 1,038 Eurasian grape varieties with unique profiles to assess the population genetic diversity, structure, and relatedness, and to infer the most likely migration events. Comparisons of putative scenarios of gene flow throughout Europe from Caucasus helped to fit the more reliable migration routes around the Mediterranean Basin. Approximate Bayesian computation (ABC) approach made possible to provide a response to several questions so far remaining unsolved. Firstly, the assessment of genetic diversity and population structure within a we…

Comparative Analysis of Machine Learning Methods to Predict Growth of F. sporotrichioides and Production of T-2 and HT-2 Toxins in Treatments with Et…

2021

The efficacy of ethylene-vinyl alcohol copolymer films (EVOH) incorporating the essential oil components cinnamaldehyde (CINHO), citral (CIT), isoeugenol (IEG), or linalool (LIN) to control growth rate (GR) and production of T-2 and HT-2 toxins by Fusarium sporotrichioides cultured on oat grains under different temperature (28, 20, and 15 °C) and water activity (aw) (0.99 and 0.96) regimes was assayed. GR in controls/treatments usually increased with increasing temperature, regardless of aw, but no significant differences concerning aw were found. Toxin production decreased with increasing temperature. The effectiveness of films to control fungal GR and toxin production was as follows: EVOH…

Integrated Bayesian Approaches Shed Light on the Dissemination Routes of the Eurasian Grapevine Germplasm

2021

The domestication and spreading of grapevine as well as the gene flow history had been described in many studies. We used a high-quality 7k SNP dataset of 1,038 Eurasian grape varieties with unique profiles to assess the population genetic diversity, structure, and relatedness, and to infer the most likely migration events. Comparisons of putative scenarios of gene flow throughout Europe from Caucasus helped to fit the more reliable migration routes around the Mediterranean Basin. Approximate Bayesian computation (ABC) approach made possible to provide a response to several questions so far remaining unsolved. Firstly, the assessment of genetic diversity and population structure within a we…

ConvLSTM Neural Networks for seismic event prediction in Chile

2021

Predicting seismic risk is a challenging task in order to avoid catastrophic effects. In this work, two models based on Convolutional Network (CNN) and Long Short Term Memory (LSTM) networks are proposed to predict the seismic risk in Chile. In particular, a ConvLSTM and a Multi-column ConvLSTM network are used for the prediction of the average number of seismic events greater than 2,8 magnitude on the Richter scale, in the Chilean regions of Coquimbo and Araucania between the years 2010 and 2017. For this model, the values of the intensity function estimated through an ETAS model and the accumulated displacement prior to a the seismic events are used as inputs. In particular, given the spa…

A predictive model for salt nanoparticle formation using heterodimer stability calculations

2021

Acid–base clusters and stable salt formation are critical drivers of new particle formation events in the atmosphere. In this study, we explore salt heterodimer (a cluster of one acid and one base) stability as a function of gas-phase acidity, aqueous-phase acidity, heterodimer proton transference, vapor pressure, dipole moment and polarizability for salts comprised of sulfuric acid, methanesulfonic acid and nitric acid with nine bases. The best predictor of heterodimer stability was found to be gas-phase acidity. We then analyzed the relationship between heterodimer stability and J4×4, the theoretically predicted formation rate of a four-acid, four-base cluster, for sulfuric acid salts ove…

L’interaction des pesticides est délétère pour les abeilles

2021

International audience

Influence of Oral Health Care Systems on Future Career Environment of Dental Students in Europe

2021

Oral healthcare is organized subsidiarily and independently by nation states in Europe and also within the EU and consequently, major differences between the nation states and the various oral healthcare systems in Europe are present. The socialization in the respective catchment area can have an impact on the job choice and the perception of employment opportunities of different professional groups. Therefore, the purpose of this survey was to elucidate the influence of different oral healthcare systems on students living or studying in the respective catchment area. A questionnaire (in English, French, German, Italian, and Spanish) with 18 different components was administered. Data on ge…

Carbon concentration increases with depth of melting in Earth’s upper mantle

2021

Carbon in the upper mantle controls incipient melting of carbonated peridotite and so acts as a critical driver of plate tectonics. The carbon-rich melts that form control the rate of volatile outflux from the Earth’s interior, contributing to climate evolution over geological times. However, attempts to constrain the carbon concentrations of the mantle source beneath oceanic islands and continental rifts is complicated by pre-eruptive volatile loss from magmas. Here, we compile literature data on magmatic gases, as a surface expression of the pre-eruptive volatile loss, from 12 oceanic island and continental rift volcanoes. We find that the levels of carbon enrichment in magmatic gases cor…

The experiences of older individuals providing care to older dependents: A phenomenological study in Spain

2021

Objective Non-professional care provided in domestic settings by a family member or someone from the close environment and without a connection to a professional care service, is increasingly assumed by older people, mainly the spouses of those requiring care. The aim of this study was to describe the experience of older people providing care at home to older dependents. Methods A qualitative study was carried out to describe and explore the experience of older people, caregivers of dependent older people in the home. Results Four themes emerged as a result of the analysis: interpersonal relationships established in the caregivers’ immediate environment; the need and request for public and…

An expanding annular plaque on the leg

2021

All-optical long-distance quantum communication with Gottesman-Kitaev-Preskill qubits

2021

Quantum repeaters are a promising platform for realizing long-distance quantum communication and thus could form the backbone of a secure quantum internet, a scalable quantum network, or a distributed quantum computer. Repeater protocols that encode information in single- or multi-photon states are limited by transmission losses and the cost of implementing entangling gates or Bell measurements. In this work, we consider implementing a quantum repeater protocol using Gottesman-Kitaev-Preskill (GKP) qubits. These qubits are natural elements for quantum repeater protocols, because they allow for deterministic Gaussian entangling operations and Bell measurements, which can be implemented at ro…

Nobiletin and xanthohumol sensitize colorectal cancer stem cells to standard chemotherapy

2021

Simple Summary Colorectal cancer stem cells (CR-CSCs) play a pivotal role in the therapy resistance and relapse of CRC patients. Herein we demonstrate that new treatment approaches comprising polymethoxyflavones and prenylflavonoids extracted from Citrus sinensis and Humulus lupulus, respectively, hamper the viability of CR-CSCs as well as synergizing with 5-fluorouracil and oxaliplatin (FOX)-based chemotherapy. Extract fractions containing Nobiletin and Xanthohumol, in combination with chemotherapy, decreased stemness properties of CR-CSCs and restrained the outgrowth of chemoresistant metastatic CR-CSCs. These data pinpoint Nobiletin and Xanthohumol as efficacious anti-cancer compounds in…

Enhanced Interplay between Host–Guest and Spin-Crossover Properties through the Introduction of an N Heteroatom in 2D Hofmann Clathrates

2021

Controlled modulation of the spin-crossover (SCO) behavior through the sorption-desorption of invited molecules is an extensively exploited topic because of its potential applications in molecular sensing. For this purpose, understanding the mechanisms by which the spin-switching properties are altered by guest molecules is of paramount importance. Here, we show an experimental approach revealing a direct probe of how the interplay between SCO and host-guest chemistry is noticeably activated by chemically tuning the host structure. Thus, the axial ligand 4-phenylpyridine (4-PhPy) in the 2D Hofmann clathrates {Fe(4-PhPy)2[M(CN)4]} (PhPyM; M = Pt, Pd) is replaced by 2,4-bipyridine (2,4-Bipy),…

Training Cardiac Surgeons: Safety and Requirements.

2021

To analyze whether cardiac surgical residents can perform their first surgeries without compromising patients' safety or outcomes, by comparing their performance and results to those of senior surgeons. All documented CABGs conducted between 2002 and 2020 were included. Surgeries were divided according to the experience level of the main surgeon (defined by the number of CABG conducted by him/her) using the following thresholds: 1000; 150; 80 and 35. This resulted in 5 groups: senior surgeons (the reference group); attending surgeons; fellow surgeons; advanced residents and new residents. Primary endpoint was 30 day mortality. Secondary endpoints included a list of intra and post-operative …

Active and Passive Motion in Complex pH‐Fields

2021

Shedding Light on the Formation and Structure of Kombucha Biofilm Using Two-Photon Fluorescence Microscopy

2021

Kombucha pellicles are often used as inoculum to produce this beverage and have become a signature feature. This cellulosic biofilm produced by acetic acid bacteria (AAB) involves yeasts, which are also part of the kombucha consortia. The role of microbial interactions in thede novoformation and structure of kombucha pellicles was investigated during the 3 days following inoculation, using two-photon microscopy coupled with fluorescent staining. Aggregated yeast cells appear to serve as scaffolding to which bacterial cellulose accumulates. This initial foundation leads to a layered structure characterized by a top cellulose-rich layer and a biomass-rich sublayer. This sublayer is expected t…

Arterial Load and Norepinephrine Are Associated With the Response of the Cardiovascular System to Fluid Expansion

2021

BackgroundFluid responsiveness has been extensively studied by using the preload prism. The arterial load might be a factor modulating the fluid responsiveness. The norepinephrine (NE) administration increases the arterial load and modifies the vascular properties. The objective of the present study was to determine the relationship between fluid responsiveness, preload, arterial load, and NE use. We hypothesized that as a preload/arterial load, NE use may affect fluid responsiveness.MethodsThe retrospective multicentered analysis of the pooled data from 446 patients monitored using the transpulmonary thermodilution before and after fluid expansion (FE) was performed. FE was standardized be…

Decellularized tracheal prelamination implant: A proposed bilateral double organ technique

2021

Introduction In tracheal replacement transplantation, prelamination is a critical stage. Nowadays, the most widely used prelamination technique is the prethoracic fascia flap with lateral thoracic artery. We propose a flap based on the internal thoracic artery, which allows a relatively non-aggressive double organ implant, and we have tested its efficacy in decellularized tracheas. Material and methods Tracheas of albino New Zealand rabbits were decellularized following a protocol that uses detergents and cryogenization, sterilized with 1kGy gamma radiation and tutorized with a stent. Bilateral pedicled flaps made of pectoral fascia and a muscular component were harvested through a longitud…

What is the accuracy of the surgical guide in the planning of orthognathic surgeries? A systematic review.

2021

BACKGROUND To investigate the true accuracy of the surgical guide in the planning of orthognathic surgeries, which are performed worldwide. MATERIAL AND METHODS A systematic search was conducted in the PubMed database, Web of science, Scopus and Embase, covering August 2020 to January 2021. Studies that included patients with dentofacial deformity including anteroposterior, vertical and asymmetry problems who were undergoing an orthognathic surgery procedure were included; QUADAS-2 was used to determine the risk of bias by analyzing the quality of the studies. A PRISMA (flowchart) was created to show the study selection, keywords, nomination processes, and inclusion and exclusion criteria. …

Molecular stabilization of chemically exfoliated bare MnPS3 layers

2021

Transition metal chalcogenophosphates of general formula MPX3 have attracted recent interest in the field of 2D materials due to the possibility of tuning their properties when reaching the 2D limit. Several works address this challenge by dry mechanical exfoliation. However, only a few of them use a scalable approach. In this work, we apply a general chemical protocol to exfoliate MnPS3. The method uses in a first step chemical intercalation and liquid phase exfoliation, followed in a second step by the addition of molecules used as capping agents on the inorganic layers. Therefore, molecules of different nature prompts the quality of the exfoliated material and its stabilization in aqueou…

Target 5G visible light positioning signal subcarrier extraction method using particle swarm optimization algorithm

2021

International audience; With the explosive growth of demand for Internet of Things (IoT) applications and the increasing dependence of users on wireless connections, indoor location based service(LBS) under 5G-Public-Private Partnership (5G-PPP) using cases have received more attention and get rapid development. Thanks to the safty, security and customization of 5G network pointed by 5G forum white paper, indoor positioning systems using unified 5G New Radio (NR) signals have become the focus of the next generation of visible light positioning (VLP) systems. In 5G New Radio(NR) frame, totally 192 subcarriers are used to carry positioning reference signal(PRS). In order to improve the positi…

The effects of physiotherapy using proprioceptive neuromuscular facilitation techniques on the gait of patients after hip and knee arthroplasty: a ca…

2021

Background: Gait disturbances are a major problem for patients after arthroplasty. After the surgery, walking speed, the rhythm of locomotion, and the length of the steps are significantly reduced. One of the therapeutic methods used in gait re-education is proprioceptive neuromuscular facilitation (PNF). Aim of the study: This study aimed to evaluate the effects of physiotherapy using PNF techniques on the gait of patients after hip and knee arthroplasty. Case report: A 60-year-old woman with advanced osteoarthritis that received bilateral hip and right knee arthroplasties was examined. Before and after the therapy, the following tests were performed: measurement of the range of motion (RO…

Patterns of Innate or Acquired Resistance to Anticancer Drugs: Our Experience to Overcome It

2021

Drug resistance, which is often of a multiple type, can be defined as the ability of cancer cells to obtain resistance to both conventional and novel chemotherapy agents. It remains a major problem to solve in cancer therapy. The mechanisms of resistance are multifactorial, and in our cellular models of acute myeloid leukemia, hepatocellular carcinoma, and triple-negative breast cancer, it involves the NF-κB pathway. In our opinion, multitarget molecules can be considered as privileged compounds capable of attacking and reversing the resistant phenotype. In the phenomena of both innate and acquired drug resistance that we have been studying since 1998 to today and up to 2016 under the guida…

Motor strategies and adiabatic invariants: The case of rhythmic motion in parabolic flights

2021

The role of gravity in human motor control is at the same time obvious and difficult to isolate. It can be assessed by performing experiments in variable gravity. We propose that adiabatic invariant theory may be used to reveal nearly-conserved quantities in human voluntary rhythmic motion, an individual being seen as a complex time-dependent dynamical system with bounded motion in phase-space. We study an explicit realization of our proposal: An experiment in which we asked participants to perform $\infty-$ shaped motion of their right arm during a parabolic flight, either at self-selected pace or at a metronome's given pace. Gravity varied between $0$ and $1.8$ $g$ during a parabola. We c…

VR Storytelling

2021

The monographic section of this issue of Cinergie arises from a need that has becoming increasingly acute in the past few years, namely that of closely examining the modes, practices, strategies, and forms of storytelling used in the various forms and experiences of Virtual Reality (VR) entertainment. The question that we asked ourselves, and thus also the eld of analysis that we wanted to open focuses primarily on two elements: first, on if and how cinematic narration can exist in VR; second, on what paths the development of a form of storytelling not directly tied to cinematic language might take.

Introduction to the special issue

2021

The article is the introduction to an Urban Research and Practice special issue conceived with the aim of exploring some of the different ways EU regional policy may have influenced the development process of four Southern European cities, Porto (PT), Malaga (ES), Palermo (IT), and Thessaloniki (GR). The selection of case studies relates to cities at the ‘margins of Europe’ in both the sense of places outside the geographical core of the continent and located in those regions – the ‘less developed regions’ – where EU Cohesion policy has been manifested in significant investment over an extended period of time. For that reason, the special issue also seeks to provide an urban perspective to …

Novel Glutamate–Putrescine Ligase Activity in Haloferax mediterranei: A New Function for glnA-2 Gene

2021

This article belongs to the Section Cellular Biochemistry.

EL TRIBUNAL ESPECIAL PARA EL LÍBANO Y SU CONTRIBUCIÓN AL FIN DE LA IMPUNIDAD: Una mirada crítica

2021

Entre los diversos tribunales penales internacionales ad hoc que se han creado desde finales del siglo XX, el Tribunal Especial para el Líbano destaca por su particularidad, tanto a nivel jurisdiccional como por su contribución al tratamiento de las víctimas. La labor del Tribunal Especial para el Líbano comenzó en 2009, dictando su primera sentencia de instancia el 18 de agosto de 2020. Pronto se podrá delimitar su eficacia como medida del Capítulo VII en el propio Líbano, pero cabe destacar el esfuerzo del Consejo de Seguridad por no abandonar el camino iniciado en 1993 con la creación de los tribunales de Yugoslavia y Ruanda. Este trabajo pretende analizar las peculiaridades del Tribunal…