Search results for "Incubation"
showing 10 items of 200 documents
Metabolism of Linoleic Acid or Mevalonate and 6-Pentyl-α-Pyrone Biosynthesis by Trichoderma Species
1993
The understanding of the biosynthetic pathway of 6-pentyl-α-pyrone in Trichoderma species was achieved by using labelled linoleic acid or mevalonate as a tracer. Incubation of growing cultures of Trichoderma harzianum and T. viride with [U- 14 C]linoleic acid or [5- 14 C]sodium mevalonate revealed that both fungal strains were able to incorporate these labelled compounds (50 and 15%, respectively). Most intracellular radioactivity was found in the neutral lipid fraction. At the initial time of incubation, the radioactivity from [ 14 C]linoleic acid was incorporated into 6-pentyl-α-pyrone more rapidly than that from [ 14 C]mevalonate. No radioactivity incorporation was detected in 6-pentyl-…
Anaerobic batch degradation of solid poultry slaughterhouse waste
2000
We studied anaerobic batch degradation of solid poultry slaughterhouse wastes with different initial waste and inoculum concentrations and waste-to-inoculum ratios and simulated the dynamics of the process with a new generation <METHANE> model. Our modelling results suggest that inhibited propionate degradation by long-chain fatty acids (LCFA) and inhibited hydrolysis by a high propionate concentration constituted the rate-limiting step in the waste degradation. Palmitate was the most abundant LCFA in the assays. Within 27 days of incubation, up to 0.55 to 0.67 m3 of methane (STP)/kg VS added was produced under the studied conditions. Lower waste-to-inoculum ratios exhibited a…
Enzyme Cytochemistry of Fungi
1973
Summary The results of a great number of investigationshave shown that enzyme cytochemical methods, which have been developed for the study of animal and human histology and cytology, can be successfully used for the study of enzyme patterns in yeasts, moulds, slime moulds, dermatophytes, phytopathogenic fungi and basidiomycetes. Whereas with the older, relatively unspecific procedures only presence or absence of enzymes could be studied, improved techniques allow the examination of the intracellular localization of enzymes under the light and especially the electron microscope. The results of those investigations are described together with methodological aspects of cultivation, incubation…
The Receptor Functions of Endogenous C1q, a Subcomponent of the First Component of Complement, on Peritoneal Macrophages
1982
Abstract C1q, the Fc recognizing subcomponent of the first complement component was shown to be synthesized by peritoneal macrophages. Evidence is presented that C1q serves during the secretion phase as Fc binding protein on the membrane of these macrophages. A dose-dependent inhibition of Fc rosette formation occured when the macrophages were pretreated with anti-C1q -F(ab') 2 . The C3b rosette formation was not affected. In addition, preincubation of peritoneal macrophages with anti-C1q -F(ab') 2 abolished specifically the polyanion mediated stimulation to secrete dose and time dependently lysosomal enzymes. There was no polyanion-induced enzyme release after incubation of polyanions with…
Virazole (Ribavirin) a Cytostatic Agent
1978
Virazole (1-β-D-ribofuranosyl-1,2,4-triazole-3carboxamide,ribavirin) is a synthetic triazol nucleoside with a broad spectrum of antiviral activity. But virazole affects the metabolism not only of virus infected cells. Virazole strongly inhibits the cell proliferation of mouse lymphoma cells (L 5178y), which were not infected with DNA- or RNA-viruses. Starting with 3 × 103 cells/ml and an incubation period of 72 hr, the drug reduces the cell proliferation to 50% (= ED50 concentration) in a concentration of 4,7 μM.
Calcium efflux from human erythrocyte ghosts
1970
The passive Ca efflux from human red cell ghosts was studied in media of differing ion compositions and compared to the ATP-dependent Ca efflux. Cells were loaded with(45)Ca during reversible hemolysis, and the loss of radioactivity into the non-radioactive incubation medium was measured, usually for 3 hr at 37°C. Analysis of the efflux curves revealed that(45)Ca efflux followed the kinetics of a simple two-compartment system. In the concentration range between 0 and 1MM Ca in the external solution ([Ca(++)] o ), the rate constant of passive Ca efflux (k min(-1), fraction of(45)Ca lost per minute into the medium) increased from 0.00732 to 0.0150 min(-1). There was no further increase at hig…
Running against time: conservation of the remaining hawksbill turtle (Eretmochelys imbricata) nesting population in the Dominican Republic
2012
Saona Island hosts the last hawksbill turtle (Eretmochelys imbricata) nesting population in the Dominican Republic, which has experienced a severe decline in the last decades, mostly due to illegal egg take. Here we present the results of an artificial incubation programme started in 2007 to protect the clutches from human predation. A preliminary survey in 2006 showed that about 60% of clutches laid were taken by humans. Over the study period (2007–2010) we recorded 400 clutches, of which 38.2% were predated by humans, 40.7% were artificially incubated and 21% were incubated in situ. Overall, the artificial incubation programme allowed the release of 12,340 hatchlings. No differences were …
Changes in the motility patterns of spermatozoa from the rabbit epididymis as assessed by computer-aided sperm motion analysis
1996
Sperm maturation in the epididymis includes changes in their potential for motility that enables spermatozoa to reach the egg and penetrate its investments. The motility characteristics of spermatozoa from the testis, the epididymis, and vas deferens of the rabbit were investigated by computer-assisted sperm analysis (CASA). Various forms of motility were displayed by sperm from different regions of the epididymis released into incubation medium. Testicular sperm were motile, although nonprogressive. The maximum percentage motility was expressed by sperm in the proximal cauda epididymidis, and forward progression was developed by spermatozoa from the distal caput. Once forward progression w…
Binding of Cu2+, Zn2+, and Ni(2+)-GnRH complexes with the rat pituitary receptor.
1997
Complex of copper with the gonadotropin-releasing hormone, GnRH, competed more efficiently for the GnRH receptor than native GVRH, while complexes of nickel with GnRH and zinc with GnRH had slightly lower affinity. Copper ion added to the incubation mixture inhibited the buserelin binding to the receptor.
Zeitlicher Ablauf der ACTH-induzierten Stimulierung der Corticosteroidbiosynthese
1969
The time course of ACTH action was studied during incubation of quartered rat adrenals by estimation of corticosterone production and secretion rates. The results suggest a two step action of ACTH during the acute stimulation of corticosteroid biosynthesis: A modest initial stimulation was observed within the first 30 min of exposure to ACTH which was overlapped by a marked secondary stimulation between 30 and 60 min.