Search results for "biosensor"

SMOOTHLY SHIFTING FLUORESCENT WINDOW: TUNABLE “OFF-ON-OFF” MICELLAR BIOSENSORS FOR pH

2009

Identification of high-affinity phage-displayed VH fragments by use of a quartz crystal microbalance with dissipation monitoring

2021

10 Pág. Departamento de Tecnología de Alimentos​​

Pathogen-Mimicking MnO Nanoparticles for Selective Activation of the TLR9 Pathway and Imaging of Cancer Cells

2009

Here, design of the first pathogen-mimicking metal oxide nanoparticles with the ability to enter cancer cells and to selectively target and activate the TLR9 pathway, and with optical and MR imaging capabilities, is reported. The immobilization of ssDNA (CpG ODN 2006) on MnO nanoparticles is performed via the phosphoramidite route using a multifunctional polymer. The multifunctional polymer used for the nanoparticle surface modification not only affords a protective organic biocompatible shell but also provides an efficient and convenient means for loading immunostimulatory oligonucleotides. Since fluorescent molecules are amenable to photodetection, a chromophore (Rhodamine) is introduced …

Novel Immune TiO2 Photoluminescence Biosensors for Leucosis Detection

2012

Abstract Novel immune photoluminescent biosensor, based on TiO2 nanoparticles, for retroviral leucosis detection has been developed. The photoluminescence spectra were excited by solid state laser with wavelength 355 nm and measured in the range of 370-800 nm. Original photoluminescence spectrum of TiO2 nanoparticles showed wide maximum at 515 nm. The biosensitive layer was formed by immobilization of retroviral leucosis antigens on the surface of TiO2 nanoparticles. Immobilization of antigens on TiO2 surface led to UV-shift of photoluminescence spectrum and increase of PL intensity. The response to different concentrations of retroviral leucosis antibodies has been measured. The decrease o…

Application of Room Temperature Photoluminescence From ZnO Nanorods for Salmonella Detection

2014

ZnO nanorods grown by gaseous-disperse synthesis are confirmed by XRD analysis to have the wurtzite crystal structure. The obtained crystallites, as found from SEM studies, are 57 +/- 9 nm in diameter and 470 +/- 30 nm long on the average. Two emission bands of photoluminescence from ZnO nanorods observed at room temperature are centered at 376 and 520 nm. A biosensitive layer is prepared by immobilization of anti-Salmonella antibodies from liquid solutions on the ZnO surface. Immobilization of the biosensitive layer onto ZnO nanorods is found to increase the intensity of PL. After further reaction with Salmonella antigens (Ags), the PL intensity is found to decrease proportional to Ag conc…

Visible Photoluminescence of Variable-Length Zinc Oxide Nanorods Embedded in Porous Anodic Alumina Template for Biosensor Applications

2021

Zinc oxide (ZnO) and porous anodic aluminum oxide (PAAO) are technologically important materials, rich with features that are of interest in optical applications, for example, in light-emitting and sensing devices. Here, we present synthesis method of aligned ZnO nanorods (NR) with 40 nm diameter and variable length in 150 to 500 nm range obtained by atomic layer deposition (ALD) of ZnO in pores of continuously variable thickness PAAO. The relative intensity of yellow (1.99 eV), green (2.35 eV), and blue (2.82 eV) photoluminescence (PL) components originating from the different types of defects, varied with non-monotonic dependency on the composite film thickness with a Fabry–Pérot like mod…

Real-time and low-cost sensing technique based on photonic bandgap structures

2011

[EN] A technique for the development of low-cost and high-sensitivity photonic biosensing devices is proposed and experimentally demonstrated. In this technique, a photonic bandgap structure is used as transducer, but its readout is performed by simply using a broadband source, an optical filter, and a power meter, without the need of obtaining the transmission spectrum of the structure; thus, a really low-cost system and real-time results are achieved. Experimental results show that it is possible to detect very low refractive index variations, achieving a detection limit below 2 x 10(-6) refractive index units using this low-cost measuring technique. (C) 2011 Optical Society of America[

Quantum dots functionalised artificial peptides bioinspired to the D1 protein from the Photosystem II of Chlamydomonas reinhardtii for endocrine disr…

2021

Herein we describe the design and synthesis of novel artificial peptides mimicking the plastoquinone binding niche of the D1 protein from the green photosynthetic alga Chlamydomonas reinhardtii, also able to bind herbicides. In particular, molecular dynamics (MD) simulations were performed to model in silico the behaviour of three peptides, D1Pep70-H, D1Pep70-S264K and D1Pep70-S268C, as genetic variants with different affinity towards the photosynthetic herbicide atrazine. Then the photosynthetic peptides were functionalised with quantum dots for the development of a hybrid optosensor for the detection of atrazine, one of the most employed herbicides for weed control in agriculture as well …

IRiS—Exploring new frontiers in neutron-rich isotopes of the heaviest elements with a new Inelastic Reaction Isotope Separator

2011

Abstract A dedicated Inelastic Reaction Isotope Separator (IRiS) for multi-nucleon transfer products will be designed and installed at GSI. Research at IRiS will focus on the investigation of new neutron-rich isotopes of the heaviest elements, study of which will advance various research fields, such as nuclear chemistry, nuclear and atomic physics, as well as nuclear astrophysics. The scientific motivation for this project and the alternative design options for the separator and its main components are discussed.

Integrated optic surface plasmon resonance measurements in a borosilicate glass substrate

2008

The surface plasmon resonance (SPR) technique is a well-known optical method that can be used to measure the refractive index of organic nano-layers adsorbed on a thin metal film. Although there are many configurations for measuring biomolecular interactions, SPR-based techniques play a central role in many current biosensing experiments, since they are the most suited for sensitive and quantitative kinetic measurements. Here we give some results from the analysis and numerical elaboration of SPR data from integrated optics experiments in a particular borosilicate glass, chosen for its composition offering the rather low refractive index of 1.4701 at 633 nm wavelength. These data regard the…