Search results for "virus"
showing 10 items of 5024 documents
Asymptomatic carotid lesion as marker of future cerebrovascular and cardiovascular events in the follow-up: correlation with markers of inflammation …
2002
Repression of Human Papillomavirus Oncogene Expression under Hypoxia Is Mediated by PI3K/mTORC2/AKT Signaling
2019
Oncogenic HPV types are major human carcinogens. Under hypoxia, HPV-positive cancer cells can repress the viral E6/E7 oncogenes and induce a reversible growth arrest. This response could contribute to therapy resistance, immune evasion, and tumor recurrence upon reoxygenation. Here, we uncover evidence that HPV oncogene repression is mediated by hypoxia-induced activation of canonical PI3K/mTORC2/AKT signaling. AKT-dependent downregulation of E6/E7 is only observed under hypoxia and occurs, at least in part, at the transcriptional level. Quantitative proteome analyses identify additional factors as candidates to be involved in AKT-dependent E6/E7 repression and/or hypoxic PI3K/mTORC2/AKT ac…
Telomere length in cervical smears of women with high-risk human papillomavirus (HPV HR)
2018
Hepatīta B kora antigēna veidoto vīrusveidīgo daļiņu modificēšana vakcīnu prototipa izstrādei
2017
DENV vakcīnu izveide balstās galvenokārt, izmantojot dzīvus novājinātus vīrusus. 2015. gadā tika reģistrēta pirmā tetravalenta novājināta denges vīrusa vakcīna. Kā drošāks vakcīnas veids ir subvienību vakcīnas. Daudzsološākais DENV antigēns subvienību vakcīnas izveidē ir apvalka proteīna III domēns. Lai palielinātu antigēna subvienības imunogenitāti, tiek izmantotas vīrusveidīgās daļiņas (VLP) kā svešu antigēnu nesēji. Tā kā VLP ir pašnereplicējošas, tās tiek uzskatītas par drošākiem kandidātiem vakcīnu izveidē, salīdzinot ar dzīviem novājinātiem vīrusiem. Maģistra darba mērķis bija iegūt 2. serotipa denges vīrusa apvalka III domēnu (DV2-III) prezentējošas mutantās HBc VLP. Pētījumā tika pā…
Polymers with antiviral properties: A brief review.
2021
Viruses that are pathogenic to humans and livestock pose a serious epidemiological threat and challenge the world's population. The SARS-CoV-2/COVID-19 pandemic has made the world aware of the scale of the threat. The surfaces of various materials can be a source of viruses that remain temporarily contagious in the environment. Few polymers have antiviral effects that reduce infectivity or the presence of a virus in the human environment. Some of the effects are due to certain physical properties, e.g., high hydrophobicity. Other materials owe their antiviral activity to a modified physicochemical structure favoring the action on specific virus receptors or on their biochemistry. Current re…
Retrovirus infection and aging: Increase in UAG suppressor tRNA expression in aged mice.
1994
Summary The effect of aging on expression of a natural glutamine suppressor tRNA (tRNA Gln(UmUG) ) was studied in different tissues of mice; this tRNA recognizes UAG and inserts glutamine at the site of the termination codon. The level of tRNA Gln(UmUG) was found to be strongly increased in aged mice, compared to newborn and mature animals. An elevated expression of tRNA Gln(UmUG) has also been found in retrovirus-infected cells; in Moloney virus-infected cells the suppressor tRNA allows to read-through the UAG codon within the retroviral protease gene. We suggest that the increase in the level of tRNA Gln(UmUG) may influence retroviral gene expression with age.
Inhibition of the herpes simplex virus-coded thymidine kinase-complex by 9-?-D-arabinofuranosyladenine 5?-monophosphate (ara-AMP) and 9-(2-hydroxyeth…
1984
The thymidine kinase-complex isolated from herpes simplex virus type 1 and type 2 (HSV-1 and HSV-2) is associated with the following enzyme activities: ATP:dThd (dCyd) deoxypyrimidine kinase, ATP:dTMP thymidylate kinase, ADP:dThd- and AMP:dThd5′-phosphotransferase. In kinetic experiments it is shown that ara-AMP inhibits AMP:dThd- and ADP:dThd phosphotransferase activity, while acyclo-GMP impairs ADP:dThd phosphotransferase reaction only; the inhibition was found to be non-compertitive. The functional subunit ATP:dThd kinase was not affected by either compound.
Structural Properties of Carnation Mottle Virus p7 Movement Protein and Its RNA-binding Domain
2001
Plant viral movement proteins (MPs) participate actively in the intra- and intercellular movement of RNA plant viruses to such an extent that MP dysfunction impairs viral infection. However, the molecular mechanism(s) of their interaction with cognate nucleic acids are not well understood, partly due to the lack of structural information. In this work, a protein dissection approach was used to gain information on the structural and RNA-binding properties of this class of proteins, as exemplified by the 61-amino acid residue p7 MP from carnation mottle virus (CarMV). Circular dichroism spectroscopy showed that CarMV p7 is an alpha/beta RNA-binding soluble protein. Using synthetic peptides de…
Possible transmission flow of SARS-CoV-2 based on ACE2 features
2020
AbstractAngiotensin-converting enzyme 2 (ACE2) is the cellular receptor for the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) that is engendering the severe coronavirus disease 2019 (COVID-19) pandemic. The spike (S) protein receptor-binding domain (RBD) of SARS-CoV-2 binds to the three sub-domains viz. amino acids (aa) 22-42, aa 79-84, and aa 330-393 of ACE2 on human cells to initiate entry. It was reported earlier that the receptor utilization capacity of ACE2 proteins from different species, such as cats, chimpanzees, dogs, and cattle, are different. A comprehensive analysis of ACE2 receptors of nineteen species was carried out in this study, and the findings propose a pos…