Substrate Specificity of Vinorine Hydroxylase, a Novel Membrane-bound Key Enzyme of Rauwolfia Indole Alkaloid Biosynthesis

1995

Anthraquinones from Ophiorrhiza pumila tissue and cell cultures

1998

We have succeeded in initiating and establishing systems of tissue and cell cultures of Ophiorrhiza pumila. Examination of the constituents of the methanol extract of the cultured calli revealed the presence of 11 anthraquinones including two new ones whose structures have been rigorously proved using advanced spectroscopic methods. These findings demonstrated a remarkable difference in the constituents between the wild plants and the callus tissue or cultured cells; the former is devoid of anthraquinones and contains a variety of camptothecin-related alkaloids whereas the latter contains a significant amount of anthraquinones and shows no indication of the presence of alkaloids after sever…

ChemInform Abstract: Structure and Synthesis of a New Indole Alkaloid, 19(S)-Hydroxy-Nb- methylraumacline, Obtained by the Biotransformation of Ajmal…

2010

Isolation of Alkaloids from Cultured Hybrid Cells of Rauwolfia serpentina*Rhazya stricta.

1996

Two monoterpenoid indole alkaloids and four β-carbolines were isolated from a hydrid cell suspension culture generated from two Apocynaceous plants, Rauwolfia serpentina Benth. and Rhazya stricta Decaisne. This indicates that the function of alkoloid biosynthesis is retained after hybrid formation and that alkaloids not previously detected in the parental plants or cell cultures are formed.

Alkaloids from Cell Cultures of Aspidosperma Quebracho-Blanco

1995

Alkaloids from Rauwolfia serpentina cell cultures treated with ajmaline

1993

Abstract A group of new alkaloids, the raumaclines, and some related alkaloids were isolated from Rauwolfia serpentina cell suspensions fed with high levels of ajmaline; their structures were determined and syntheses developed providing an essential prerequisite to the further study of their biosynthesis at the enzymatic level.

Novel indole alkaloids from cell suspension cultures of aspidosperma quebracho blanco schlecht

1991

Abstract Two novel monoterpenoid indole alkaloids, aspidochibine ( 1 ) and 3-oxo-14,15-dehydrorhazinilam ( 2 ), were isolated from cell suspension cultures of Aspidosperma quebracho blanco Schlecht. Aspidochibine ( 1 ) exhibits a new alkaloid skeleton. The structure of these alkaloids was elucidated by spectroscopic methods.

Structure and Synthesis of a New Indole Alkaloid, 19 (S)-Hydroxy-Nb-methylraumacline, Obtained by the Biotransformation of Ajmaline in Plant Cell Cul…

1992

Abstract From the plant cell suspension cultures of Tauwolfia serpentina Benth ., which were cultivated in the alkaloid-production medium after feeding of ajmaline (1) , a new indole alkaloid 19- ( S )-hydroxy- N b -methylraumacline ( 4 ) was isolated. The structure of 4 first elucidated by spectroscopic analysis was determined by the chemical synthesis from ajmaline ( 1 ).

Isolation, Identification, and Chemical Synthesis of 6α-Hydroxyraumacline: A Novel Alkaloid from CultivatedRauwolfia serpentinaCells

1992

From RAUWOLFIA SERPENTINA cells cultivated in the presence of ajmaline ( 2) the new indole alkaloid, 6alpha-hydroxyraumacline ( 1), was isolated. This alkaloid also occurs in significant amounts in the nutrition medium. A simple chemical synthesis of 1 was developed starting from ajmaline ( 2).

Constituents of regenerated plants of Ophiorrhiza pumila; formation of a new glycocamptothecin and predominant formation of (3R)-deoxypumiloside over…

1997

Abstract The regeneration of plantlets was successful from Ophiorrhiza pumila callus cultures, from which a new glucosyloxy camptothecin, 9-β-glucosyloxycamptothecin, together with 15 metabolites including six camptothecin-related alkaloids was isolated. (3S)-Deoxypumiloside, one of the plausible biogenetic precursors of camptothecin, could not be found, whereas the (3R) epimer was isolated from the regenerated plants.