Search results for "Biological activity"

showing 10 items of 465 documents

The biological activity of bacteriophage DNA, prepared by the cationic detergent dilution technique

1975

Abstract The preparation of phage lambda DNA infecting E. coli K 12 with cationic detergent is described. This DNA infects E. coli spheroblasts with the same efficiency as DNA prepared by phenol methods.

DetergentsViral Plaque AssayBiologyVirus Replicationmedicine.disease_causeColiphagesBacteriophagechemistry.chemical_compoundEscherichia coliGeneticsmedicinePhenolEscherichia coliVirus quantificationDNA VirusesBiological activityLambda phageChromatography Ion Exchangebiology.organism_classificationMolecular biologyQuaternary Ammonium CompoundschemistryBiochemistryViral replicationDNA ViralDNANucleic Acids Research
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Neue chemotherapeutisch-aktive 4-trifluormethylpyrimidine

1993

Abstract Condensation of N -(2-hydroxyethyl)- N -methylguanidine sulfate with various β-diketonesbearing 1,1,1-trifluoromethyl substituents leads to 2-[ N -(2-hydroxyethyl)-methylamino]-4-trifluoromethylpyrimidine derivatives. These compounds exhibit antimycotic, trichomonazide and anti-HIV properties.

DiketoneOrganic ChemistryCondensationHuman immunodeficiency virus (HIV)Biological activitymedicine.disease_causeBiochemistryInorganic Chemistrychemistry.chemical_compoundchemistrymedicineMethylguanidineEnvironmental ChemistryOrganic chemistryPhysical and Theoretical ChemistrySulfateJournal of Fluorine Chemistry
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Antimykotische wirkstoffe. XX . Fluorierte 2-(4-toluidino)pyrimidine

1985

Aus der Kondensation von 4-Tolylguanidin (1) mit den β-Diketonen 2a-f gehen die fluorierten 2-(4-Toluidino)pyrimidine 3a-f hervor. Strukturtyp 3 zeichnet sich durch antimykotische Wirkung aus. Condensation of 4-tolylguanidine (1) with the β-diketones 2a-f yields the fluorinated 2-(4-toluidino)pyrimidines 3a-f. Structures of type 3 exhibit antimycotic activity.

Diketonechemistry.chemical_compoundPyrimidinechemistryStereochemistryOrganic ChemistryCondensationBiological activityJournal of Heterocyclic Chemistry
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The new 5- or 6-azapyrimidine and cyanuric acid derivatives of L-ascorbic acid bearing the free C-5 hydroxy or C-4 amino group at the ethylenic space…

2011

Abstract We report on the synthesis of the novel types of cytosine and 5-azacytosine (1–9), uracil and 6-azauracil (13–18) and cyanuric acid (19–22) derivatives of l -ascorbic acid, and on their cytostatic activity evaluation in human malignant tumour cell lines vs. their cytotoxic effects on human normal fibroblasts (WI38). The CD spectra analysis revealed that cytosine (5 and 6), uracil (14–16), 6-azauracil (17) and cyanuric acid (21) derivatives of l -ascorbic acid bearing free amino group at ethylenic spacer existed as a racemic mixture of enantiomers, whereas L-ascorbic derivatives containing the C-5 substituted hydroxy group at the ethylenic spacer were obtained in (4R, 5S) enantiomer…

Double bondStereochemistryAscorbic AcidCrystallography X-Ray010402 general chemistry01 natural sciencesCell LineCytosineInhibitory Concentration 50Structure-Activity Relationship03 medical and health scienceschemistry.chemical_compound0302 clinical medicineDrug DiscoveryHumansUracilta116Pharmacologychemistry.chemical_classificationTriazinespyrimidine and cyanuric acid derivatives; L-ascorbic acid; circular dichroism; cytostatic activity evaluation; X-ray diffractionOrganic ChemistryAbsolute configurationHydrogen BondingStereoisomerismUracilBiological activityHep G2 CellsGeneral MedicineFibroblastsCytostatic AgentsAscorbic acidpyrimidine and cyanuric acid derivatives ; L-ascorbic acid ; circular dichroism ; cytostatic activity evaluation ; X-ray diffraction ; cell cycle analysis0104 chemical sciences3. Good healthchemistry030220 oncology & carcinogenesisS Phase Cell Cycle CheckpointsMCF-7 CellsCyanuric acidCytosineLactoneHeLa Cells
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Intracellular retention of ABL kinase inhibitors determines commitment to apoptosis in CML cells

2012

PLoS one 7(7), e40853 (2012). doi:10.1371/journal.pone.0040853

Drugs and DevicesDrug Research and DevelopmentTime Factorsmedicine.drug_classChronic Myeloid LeukemiaIntracellular Spacelcsh:MedicineApoptosisPharmacologyPiperazinesTyrosine-kinase inhibitorHematologic Cancers and Related DisordersCell Line TumorLeukemia Myelogenous Chronic BCR-ABL Positivehemic and lymphatic diseasesLeukemiasmedicineHumansAnnexin A5Proto-Oncogene Proteins c-abllcsh:ScienceProtein Kinase InhibitorsMyeloproliferative DisordersMultidisciplinaryABLDose-Response Relationship DrugCaspase 3Chemistrylcsh:RBiological activityImatinibHematologyrespiratory tract diseasesDasatinibKineticsPyrimidinesImatinib mesylatePharmacodynamicsBenzamidesImatinib MesylateMedicineATP-Binding Cassette Transporterslcsh:QDrug Screening Assays AntitumorSignal transductionIntracellularResearch ArticleSignal Transductionmedicine.drug
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Furostanol saponins and ecdysones with cytotoxic activity from Helleborus bocconei ssp. intermedius

2009

Two furostanol saponins helleboroside A (1) and helleboroside B (2) were isolated from the methanol extract of Helleborus bocconei Ten. subsp. intermedius (Guss.) Greuter and Burdet, along with the furospirostanol saponin 4 and two ecdysones: ecdysterone (5) and polypodyne B (6). Compound 2 was enzymatically hydrolysed to give product 3. The biological activity of all compounds was tested against rat C6 glioma cells showing a significant cytotoxicity for compounds 3, 4 and 6. Copyright © 2009 John Wiley & Sons, Ltd.

EcdysoneStereochemistryHelleborusSaponinRanunculaceaePharmacognosyCell Linefurostanol saponinC6 glioma cellAnimalsSettore BIO/15 - Biologia FarmaceuticaMedicinal plantsCytotoxicitycytotoxic activityPharmacologychemistry.chemical_classificationMolecular StructureTraditional medicinebiologyPlant ExtractsGlycosideBiological activitySettore CHIM/06 - Chimica OrganicaSaponinsbiology.organism_classificationAntineoplastic Agents PhytogenicRatsHelleborusSterolschemistryH. bocconei subsp. intermediuRanunculaceae
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Effect of a Killer Toxin of Yeast on Eucaryotic Systems

1988

The Saccharomyces cerevisiae killer toxin KT 28, which inhibits sensitive yeasts, was shown to have no effect on several pathogenic fungi or on the protozoan Trichomonas vaginalis. At concentrations of about 0.1 mg/ml, a partial inhibition of the skin pathogenic fungi Trichophyton rubrum and Microsporum canis was observed at pH 6.5. No pharmacological activity was detected in various tests with several animal organs.

EcologybiologyToxinSaccharomyces cerevisiaeBiological activitymacromolecular substancesTrichophyton rubrumApplied Environmental and Public Health Microbiologymedicine.disease_causebiology.organism_classificationApplied Microbiology and BiotechnologyYeastMicrobiologymedicineTrichomonas vaginalisMicrosporum canisCytotoxicityFood ScienceBiotechnologyApplied and Environmental Microbiology
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Eight-membered heterocycles with two heteroatoms in a 1,2-relationship of interest in medicinal chemistry

2021

Abstract This chapter treats 1,2-diheterocines, eight membered heterocyclic systems with two heteroatoms, N, O and S, in an 1,2 relationship, exhibiting biological properties and exhaustively covers the literature from 2007 to the end of November 2019. In this period of time 1,2-diazocine, 1,2-oxazocine, 1,2-dioxocin and 1,5-dithiocin ring systems had derivatives exhibiting pharmacological activities. No articles on biological properties have been reported for 1,2-thiazocines and 1,2-oxathiocins. Besides uncondensed derivatives, 1,2-diheterocines fused with five-, six-, and seven-membered carbocycles or heterocycles have been reviewed, as well as bridged 1,2-diheterocines.

Eight-membered heterocycles with two heteroatoms 12 Medicinal chemistry Biological activity 12-Diazocines 12-Oxazocines 12-Dioxocins 12-DithiocinsChemistryBiological propertyHeteroatomOrganic chemistryRing (chemistry)
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An allergen-polymeric nanoaggregate as a new tool for allergy vaccination.

2014

Parietaria pollen is one of the major causes of allergic reaction in southern Europe, affecting about 30% of all allergic patients in this area. Specifi immunotherapy is the only treatment able to modify the natural outcome of the disease by restoring a normal immunity against allergens. The preparation of allergen-solid lipid nanoparticles as delivery vehicles for therapeutic proteins, P. judaica major allergen Par j 2, was investigated. The Par j 2 allergen was expressed in a large amount in Escherichia coli and purifid to homogeneity. Its immunological properties were studied by western blotting and enzyme-linked immunosorbent assay inhibition. Solid lipid nanoparticles were obtained by …

ElectrophoresisLightCell SurvivalChemistry PharmaceuticalPharmaceutical ScienceImmunoglobulin Emedicine.disease_causeMicroscopy Atomic ForceHemolysislaw.inventionCell LineMiceAllergenDrug StabilitylawZeta potentialmedicineSide chainHypersensitivityAnimalsHumansNanotechnologyScattering RadiationTechnology PharmaceuticalPlant ProteinsDrug CarriersVaccines SyntheticbiologyChemistryMacrophagesVaccinationBiological activityAllergensAntigens PlantImmunoglobulin EIn vitroBasophilsElectrophoresisAllergyParietaria pollenRecombinant allergens PHEAPolymeric nanoaggregatesBiochemistryImmunologybiology.proteinRecombinant DNANanoparticlesPeptides
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Synthesis and biological activity of novel sialyl-lewisX conjugates

1996

Abstract Novel sialyl Lewis X conjugates have been synthesized and evaluated as inhibitors of E- and P-selectin mediated cell adhesion in cell culture assays. The most potent conjugate in the static inhibition assays exhibited a significant and dose-dependent pharmacological potency as inhibitor of the edotoxin-induced leukocyte adhesion to the endothelium of postcapillary venules in rats.

EndotheliumOrganic ChemistryClinical BiochemistryPharmaceutical ScienceBiological activityAdhesionBiochemistrychemistry.chemical_compoundSialyl-Lewis Xmedicine.anatomical_structurechemistryBiochemistryDrug DiscoverymedicineMolecular MedicinePotencyCell adhesionMolecular BiologyConjugateBioorganic & Medicinal Chemistry Letters
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