Search results for "ISIA"
showing 10 items of 1032 documents
Volatile components from flower-heads of Centaurea nicaeensis All., C. parlatoris Helder and C. solstitialis L. ssp. schouwii (DC.) Dostál growing wi…
2008
The volatile constituents of the flowerheads of Centaurea nicaeensis All., C. parlatoris Helder and C. solstitialis L. ssp. schouwii (DC.) Dostal were extracted by hydrodistillation and analysed by GC and GC-MS. Altogether 113 components were identified. Fatty acids and hydrocarbons were the most abundant components in the oils. Caryophyllene and caryophyllene oxide were the main compounds of the sesquiterpene fraction. The study on the biological activity of the oils shows no significant activity.
Complete decontamination and regeneration of DNA purification silica colum
2008
Silica columns are among the most used DNA purification systems, allowing a good yield of high-quality nucleic acids without organic extractions. Silica column regeneration protocols reported up to now to remove DNA traces are time-consuming, and their effectiveness on genomic DNA has not been demonstrated. Here we report a very rapid regeneration procedure that ensures no DNA carryover, independent of its size, without impairing column efficiency. The method takes advantage of the improved DNA removal by low concentrations of Triton X-100.
Staining mitochondria in Saccharomyces cerevisiae.
1969
After testing various procedures (amidoblack 10B, acid fuchsin-methyl blue, Luxol fast blue MBS-phloxine, toluidine blue O, Jams green B and pinacyanol), three stains can be recommended for staining both types of mitochondria (globose and threadlike) in the cells of Saccharomyces cerevisiae: (1) 0.1% solution of amidoblack 10B in citrate buffer (pH 3.0) for 10 min; (2) 0.01% solution of toluidine blue O in phosphate buffer (pH 6.0) for 30 min; (3) 0.01% solution of Janus green B in distilled water (pH 5.6) for 30 min. The latter stain is most specific because its staining reaction depends upon the action of the mitochondrial enzyme cytochrome c oxidase. Yet, low concentrations and short inc…
A rapid spectrofluorimetric method for the determination of the degree of synchronism inSaccharomyces cerevisiae
1985
A fluorescence technique that allows direct measurement of DNA and synchrony levels inSaccharomyces cerevisiae cell cultures is reported. The spectrofluorimetric estimation of DNA with 4,6-diamidino-2-phenylindole (AT-dye) does not require prior extraction, is highly stable, and requires small quantities of cells.
Fractionation of glycoside aroma precursors in neutral grapes. Hydrolysis and conversion by Saccharomyces cerevisiae
2004
Abstract Glycoconjugated aroma precursors from neutral grape varieties were fractionated on 5 g C18 RP cartridge. Each of the seven fractions collected was divided in three parts: first part was hydrolysed with Pectinol in order to determine total aromatic heterosides, while the second part was treated with an active strain of the yeast Saccharomyces cerevisiae UCLM 325 and the third part with acetonic powder of the same yeast strain. The studied strain proved effective in the hydrolysis of the heterosides of nerol and geraniol, cis-8-hydroxy-linalool, 8-hydroxy-dihydro-linalool, some benzenoids and some norisoprenoids derivatives such as vomifoliol and 3-oxo-α-ionol. Only whole cells are a…
The beautiful Yvonne of Louisiana. La bella Yvonne della Louisiana
2019
To understand New Orleans, it is necessary to look at her geographically: wedged on the majestic delta of the powerful Mississippi. Like any other city sitting on river deltas, her relationship with water is not linear (longitudinal as in coastal cities, transversal as in fluvial cities); rather it is complex and deep. Water appears where you least expect it, sweet and salty at the same time like her food, precious and painful like her story, with a beginning and end like her parades.
Les commissions coloniales rattachées au Comité des Travaux historiques et scientifiques
2000
The colonial Commissions connected to the Comité des travaux historiques et scientifiques. In the late nineteenth century, the Comité des travaux historiques et scientifiques became a bigger and bigger research organization, always closely linked to the successive political governments. With each new French conquest, a commission of parisian scientists and local correspondents was created and attached to it, as in 1864, the scientific commission for Mexico, in 1884, the commission for Tunisia, in 1908, the archeological commission for Indochina. The example studied here is that of the commssion for Tunisia, which coordonated researches in archeology and epigraphy in North Africa, thanks to …
Saccharomyces cerevisiae signature genes for predicting nitrogen deficiency during alcoholic fermentation
2007
Genome-wide analysis of the wine yeast strain Saccharomyces cerevisiae PYCC4072 identified 36 genes highly expressed under conditions of low or absent nitrogen in comparison with a nitrogen-replete condition. Reverse transcription-PCR analysis for four of these transcripts with this strain and its validation with another wine yeast strain underlines the usefulness of these signature genes for predicting nitrogen deficiency and therefore the diagnosis of wine stuck/sluggish fermentations.
The molecular characterization of new types of Saccharomyces cerevisiae × S. kudriavzevii hybrid yeasts unveils a high genetic diversity.
2012
11 pages, 2 tables, 4 figures. --Article first published online: 6 JAN 2012. --This is the pre-peer reviewed version of the following article: Peris, D., Belloch, C., Lopandić, K., Álvarez-Pérez, J. M., Querol, A. and Barrio, E. (2012), The molecular characterization of new types of Saccharomyces cerevisiae × S. kudriavzevii hybrid yeasts unveils a high genetic diversity. Yeast, 29: 81–91. which has been published in final form at http://dx.doi.org/10.1002/yea.2891
Ecology of yeasts associated with kernels of several durum wheat genotypes and their role in co-culture with Saccharomyces cerevisiae during dough le…
2021
International audience; This work was performed to investigate on the yeast ecology of durum wheat to evaluate the interaction between kernel yeasts and the commercial baker's yeast Saccharomyces cerevisiae during dough leavening. Yeast populations were studied in 39 genotypes of durum wheat cultivated in Sicily. The highest level of kernel yeasts was 2.9 Log CFU/g. A total of 413 isolates was collected and subjected to phenotypic and genotypic characterization. Twenty-three yeast species belonging to 11 genera have been identified. Filobasidium oeirense, Sporobolomyces roseus and Aureobasidium pullulans were the species most commonly found in durum wheat kernels. Doughs were co-inoculated …