PRELIMINARY STUDY OF “NERO D'AVOLA” CULTIVAR FROM MUST TO WINE BY SPME-GC/MS ANALYSIS

Preliminary results of a GC-MS analysis on some embalming materials from a female mummy from ptolemaic age.

Fruit physical, chemical and aromatic attributes of early, intermediate and late apricot cultivars.

BACKGROUND: In order to reach good fruit quality, apricots require a balance of sugars and acids as well as a strong apricot aroma. In this study, fruit quality of early, intermediate and late apricot cultivars was evaluated by measuring physical, chemical and olfactory attributes. Multivariate analysis of quality and aroma attributes was used to identify groups of similar cultivars and association with ripening season. RESULTS: Physical, chemical and aromatic attributes showed great variation amongcultivars but no relation to ripening season. Aromatic profiles (34 volatiles) of fruit tissues indicated qualitative and quantitative differences among cultivars. Ninfa and Mandorlon were riches…

Qualità e profilo aromatico dei frutti di Annurca sottoposti ad arrossamento in melaio

Composition and volatile aromatic fraction of "fine amber" Marsala wines

Comparison of aroma compounds from six ancient sicilian apple cultivars

Ceremonial customs of Greek age (5th Century, bC) in Sicily: a study by GC-MS of some fictile unguentaria from two necropolises

Effetto del PRD (Partial Rootzone Drying)sulla composizione della frazione volatile di mele ‘Pink Lady’

Fruit quality and volatile fraction of ‘Pink Lady’ apple trees in response to rootstock vigor and partial rootzone drying

BACKGROUND: Partial rootzone drying (PRD) is a novel deficit irrigation technique consisting in the alternated wetting of only one side of the rootzone, which induces partial stomatal closure and increased water use efficiency. The effect of PRD and rootstock vigor on ‘Pink Lady’ apple fruit quality and aroma profile was studied using solid-phase micro-extraction in headspace and gas chromatography/mass spectrometry. RESULTS: PRD irrigation generally did not affect quality attributes, whereas it influenced the aroma of the apple fruit. In particular, PRD improved the aroma of the fruit flesh, while it decreased the volatile fraction in the peel, where most of the compounds are concentrated.…

STUDY OF VOLATILE COMPONENTS OF TEN VARIETIES OF SALVIA BY SPME-GC/MS AND GENERAL DISCRIMINANT ANALYSIS

Consolidamento di legni degradati tramite polimerizzazione in situ di isoeugenolo

Indagini analitiche su campioni di verdure esposti al traffico veicolare

Hydrogen-carbon, carbon-carbon double rearrangement induced by proximity effects. 1-formation of methoxybenzyl ions in the electron impact mass spectra of substituted 1,1-bis(dimethoxyphenyl)methanes

The 75 eV electron impact mass spectra of 1,1-bis(dimethoxyphenyl)methanes bearing o-methoxy groups are dominated by intense peaks corresponding, at least formally, to benzyl ions [(CH3O)2C6H3CH2]+(b). They arise from ions [((CH3O)2C6H3)2CH]+(a), which are in turn formed from molecular ions by loss of an alkyl radical through benzylic cleavage. The analysis of compounds labelled with 2H or 13C at methoxy groups led to the determination of the mechanism. Hydrogen migration, as hydride, followed by electrophilic substitution by the methylene carbon of the phenyl methylene ether cation through a six-centred transition state is responsible for the formation of benzylic ions b.

Mass spectrometry of 3,4-dihydroquinazol-4-ones of pharmaceutical interest. Part 2: Common structure of the C8H4N2R+ ions from 3-isoxazolyl-3-pyrazolyl-, and 3-o-tolyl-2-R-substituted derivatives.

VOCS DETERMINATION IN GROUND WATERS BY SPME/GC-MS

Influence of roasting and different brewing processes on ochratoxin A content in coffee determined by high-performance liquid chromatography-fluorescence detection (HPLC-FLD)

A rapid and reliable procedure has been developed for the determination of ochratoxin A (OTA) in green and roasted coffee. The method consists of extraction of the sample with methanol-5% aqueous sodium hydrogen carbonate/1% PEG8000 (20:80), followed by immunoaffinity column (IAC) clean-up and, finally, high-performance liquid chromatography (HPLC) determination with fluorimetric detection. Mean recoveries for green and roasted coffee spiked at different levels ranging from 94 and 105% were obtained. The limit of determination (S/N = 3) was 0.032 ng g(-1) and the precision (within-laboratory relative standard deviation) was 6%. The method described has been used to assess the influence of r…

Synthesis of 1H-imidazo[3′,4′:4,5]thieno[2,3-b]pyridines. A new ring system

A facile and convenient synthesis of the title compounds is described, using as starting materials the 2-nitro-3-aminothieno[2,3-b]pyridines. Mass spectral data of the above mentioned compounds are briefly discussed.

Determination of terpene alcohols in Sicilian Muscat wines by HS-SPME-GC-MS.

Muscat is a grape family used to obtain several sweet, aromatic white dessert wines common in the Mediterranean area. Currently, three Sicilian cultivars (all classified DOC) are known: 'Moscato di Siracusa' the oldest and very rare today; 'Moscato di Noto', a modern derivative of the first and finally 'Moscato di Pantelleria', now the most common. This study concerns the volatile profile of 15 different Sicilian Muscat wines produced in different years using HS-SPME-GC-MS. In particular, four fundamental terpene alcohols (linalool, geraniol, nerol and citronellol) were considered. The principal aim was to study the evolution of aromatic compounds in wine during aging, and the information o…

Identification of organic compounds in fictile unguentaria from two Sicilian necropolis of Greek age (5th century, b.c.) by GC-MS analysis.

A study to obtain more knowledge on funeral set in Greek age, (5th Century, b.C.) was carried out on thirteen ancient unguentaria, small vessels used as containers of balms or ointments, founded in two different Sicilian necropolis: Adranon and Hymera. Every find was subjected to three extractions by increasing polarity solvents. All crude extracts, unsaponifiables and methyl esters of saponifiable fraction were analysed by GC-MS. Analysis showed difference between two groups of finds: the unguentaria from Adranon show abundant traces of lipids used in balm making, while those from Hymera resulted empty and buried for ritual purpose. Even if in the two towns, flourished in the same period, …

Comparative study on volatile compounds and FAMEs profile from sicilian monovarietal virgin oils

Studies in organic mass spectrometry.VIII. The electron impact mass spectra of 2,4-substituted-3-diazo-5-phenylpyrroles

The electron impact mass spectra (75 eV) of the β-diazopyrroles always show the molecular ions and undergo as the main fragmentation process the elimination of nitrogen followed by ring opening reactions leading to benzonitrile either as neutral or charged species. The peaks at 26 amu below the molecular ions, which are a general feature of these spectra, are due to the presence of the corresponding pyrroles which are formed by reductive reactions during the vaporization process of the samples.

Aroma compounds in sicilian olive oils from three cultivars at different ripening stages

EFFECTS OF GAMMA IRRADIATION ON THE ALFA TOCOPHEROL AND FATTY ACIDS CONTENT IN RAW UNPEELED ALMOND KERNELS (PRUNUS DULCIS)

Composti polimerici per il consolidamento di legni degradati

γ-irradiation as a methods for decontaminating food: reduction/elimination of mycotoxins in raw unpeeled almond kernels (Prunus dulcis)

Strengthening of waterlogged wood. Impregnation and polymerization within the wood EMA/MA and AIBN.

DOC Marsala: tecnologia, profilo analitico ed aromatico del Marsala Fine

Determination by solid-phase microextraction/gas chromatography/mass spectrometry of polycyclic aromatic hydrocarbons in bitumen fumes during road paving

Studio comparativo quali-quantitativo sul profilo aromatico di vini D.O.C. Marsala “Fine”

Determination of terpenes alchols in Sicilian muscat wines by HS-SPME-GC-MS.

Applicazioni di spettrometria di massa in campo alimentare: analisi di olii di oliva tramite HPLC-Ms e HS-SPME-GC-MS

A new general fragmentation reaction in mass spectrometry: The hydrogen-carbon, carbon-carbon double rearrangement of 2 heteroalkyl substituted diphenylmethyl cations

Diphenylmethyl cations formed by benzylic cleavage of the molecular ions of ortho heteroalkyl substituted 1,1-diphenylalkanes undergo the double rearrangement process (H to C followed by C to C) previously reported for ortho-methoxy derivatives. Hence the formation of substituted benzyl (or tropylium) ions allowing this double rearrangement process constitutes an interesting type of fragmentation reaction characteristic for 1,1-diphenylalkanes bearing ortho substituents (OMe, OEt, OiPr, SMe, NHMe, NMe2) which are able to transfer a hydride to the charged benzyl carbon of diphenylmethyl cations formed by benzylic cleavage of the molecular ion.

orthoeffect on the nitrosation of the 2,3-diphenyl-5(2-methoxyphenyl)pyrrole

A comparison of the behaviour on the nitrosation of the isomers 5-(2-methoxyphenyl)- (1a) 5-(3-methoxyphenyl)- (1b) and 5-(4-methoxyphenyl)2,3-diphenylpyrroles (1c) carried out in the usual way with iso-amyl nitrite in a solution of sodium ethoxide in ethanol evidences that 1a is dramatically less reactive with respect to 1b and 1c. The different reactivity was ascribed to the occurrence of a strong hydrogen bond involving pyrrole NH and the ortho-methoxy group.

Consolidamento con polimeri di reperti lignei provenienti da siti sommersi

Melatonin: Structural characterization of its non-enzymatic mono-oxygenate metabolite

Oxidation of melatonin by Fenton reagents as well as with hypochlorous acid or oxoferryl hemoglobin has been investigated. Analysis of products by low resolution/mass spectra (MS), high resolution/MS, 1H-nuclear magnetic resonance (NMR), 13C-NMR, correlated spectroscopy (COSY) and heterocorrelated spectroscopy (HETCOR) 2D NMR reveals the formation of a single mono-oxygenated product under all conditions and unequivocally assigns the N-[2-(5-methoxy-2-oxo-2,3-dihydro-1H-indol-3-yl)-ethyl]-acetamide structure, which had not been previously considered.

Studio tramite SPME-GC/MS sull'influenza della tecnologia di vinificazione e di affinamento sulla frazione volatile di vini rossi siciliani della cultivar Perricone.

Some Observations on Recent Studies Concerning the Electron Ionization Mass Spectrometry of 4(3H)-Quinazolinones

Gas chromatographic/mass spectrometric and microbiological analyses on irradiated chiken

Ionizing radiation is widely used as treatment technique for food preservation. It involves among others reduction of microbial contamination, disinfestations, sprout inhibition and extension of shelf life of food. However, the commercialization of irradiated food requires the availability of reliable methods to identify irradiated foodstuffs. In this paper, we present results on the application to irradiated chicken of this method, based on the detection, in muscle and skin samples, of the peaks of ions 98 Da and 112 Da, in a ratio approximately 4:1, typical of radiation induced 2-dodecylcyclobutanones (2-DCB). Aim of the work was also to study the time stability of the measured parameters…

Indagine tramite SPME/GC-MS su tracce d'inquinanti all'interno dell'Orto Botanico di Palermo.

Studies by SPME-GC/MS on aroma compounds of sixteen different apricot (Prunus armeniaca L.) cultivars

Characterization of glyceridic components of Sicilian extra virgin olive oils using LC/MS and L.D.A.

Mass spectrometry for cultural heritage knowledge: gas chromatographic/mass spectrometric analysis of organic remains in Neolithic potsherds

Comparative survey on nine Pistacchio curtivars grown in Sicily regarding their volatile fraction and fatty acids content

Analysis of furan in coffee of different provenance by head-space solid phase microextraction gas chromatography-mass spectrometry: effect of brewing procedures.

A simple, sensitive and accurate method for the analysis of furan in roasted coffee has been used based on headspace-solid-phase micro-extraction (HS-SPME) coupled to gas chromatography-mass spectrometry (GC-MS). The extraction was performed using 75-microm carboxen/polydimethylsiloxane fiber. Ionic strength, extraction time and temperature, and desorption time were assessed as the most important parameters affecting the HS-SPME procedure and d(4)-furan was used as the internal standard. The linearity range was in the range 0.0075-0.486 ng g(-1); the LOD and LOQ calculated using the signal-to-noise ratio approach were 0.002 and 0.006 ng g(-1), respectively. The inter- and intra-day precisio…

High Performance Liquid Chromatografy-Mass Spectrometry based chemometric characterization of olive oils

In this study the effective discrimination of extra virgin olive oils is described using HPLC-MS, combined with chemometric evaluation. The presented method is simple since the diluted oil sample is directly injected into the system, without any preliminary chemical derivatization or purification step. Separation of diacylglycerols, triacylglycerols and sterols occurs within 20 min and is achieved using an octadecyl-silica column. Detection is performed by positive APCI mass spectrometry which provided sensitivity to detect over 50 compounds in the sample. After extraction of data, stepwise discriminant function analysis is used to select the variables with the highest discriminative power.…

Studio sulle caratteristiche aromatiche di mele “Pink Lady” su due diversi portainnesto e diverso regime idrico.

Some Mass Spectrometry Contributions in the Study of Cultural Heritage

In the last decades the application of various scientific methods and technologies to study materials and handicrafts of historical, artistic or archaeological interest have dramatically increased. These studies contribute to successfully solve various problems of identification, dating, utilization of artistically or historically important objects.

Indagini tramite HS-SPME/GC-MS su prodotti di degrado di vari consolidanti per legni archeologici di alcuni reperti trattati.

A simple High Performance Liquid Chromatography-Mass spectrometry approach combined with Linear Discriminant Analysis for the characterization of top quality olive oils

Polimerizzazione in situ per il consolidamento di materiali lignei attraverso tecniche ambientalmente compatibili.

A fast liquid chromatography-mass spectrometry method combined with linear discriminant analysis for the characterization of extra virgin sicilian olive oils”

Aroma of green sicilian table olive under different irrigation treatments by SPME-GC/MS

Fruit physical, chemical and aromatic attributes of early, intermediate and late apricot cultivars

BACKGROUND: In order to reach good fruit quality, apricots require a balance of sugars and acids as well as a strong apricot aroma. In this study, fruit quality of early, intermediate and late apricot cultivars was evaluated by measuring physical, chemical and olfactory attributes. Multivariate analysis of quality and aroma attributes was used to identify groups of similar cultivars and association with ripening season. RESULTS: Physical, chemical and aromatic attributes showed great variation among cultivars but no relation to ripening season. Aromatic profiles (34 volatiles) of fruit tissues indicated qualitative and quantitative differences among cultivars. Ninfa and Mandorlon were riche…

Analysis of furan in toasted coffee by HS/SPME-GC-MS

Fruit quality traits of six ancient apple Sicilian cultivars.

ChemInform Abstract: ortho Effect on the Nitrosation of the 2,3-Diphenyl-5-(2-methoxyphenyl)pyrrole.

A comparison of the behaviour on the nitrosation of the isomers 5-(2-methoxyphenyl)- (1a) 5-(3-methoxyphenyl)- (1b) and 5-(4-methoxyphenyl)2,3-diphenylpyrroles (1c) carried out in the usual way with iso-amyl nitrite in a solution of sodium ethoxide in ethanol evidences that 1a is dramatically less reactive with respect to 1b and 1c. The different reactivity was ascribed to the occurrence of a strong hydrogen bond involving pyrrole NH and the ortho-methoxy group.

Determination of the cultivar and aging of Sicilian olive oils using HPLC-MS and linear discriminant analysis

A large number of certified samples (84) of Sicilian olive oils arising from the eight cultivars most represented in Sicily (Biancolilla, Cerasuola, Moresca, Nocellara del Belice, Nocellara Etnea, Oglialora Messinese, Brandofino and Tonda Iblea) have been collected and analyzed by HPLC/MS using an atmospheric pressure chemical ionization (APCI) source. The sample preparation is very simple; in fact, the oil samples are diluted without any chemical derivatization. A following statistical data treatment by general discriminant analysis (GDA) allows the determination of the olive oil cultivar. Furthermore, changes in the composition of glyceridic components of the olive oils lead to easy discr…

Studies in organic mass spectrometry. Part 9—mass spectra of 9,10-disubstituted 2,3,6,7-tetraalkoxy-9,10-dihydroanthracenes: A remarkable loss of radicals from even-electron ions

The electron impact-induced fragmentations of 23 9,10-disubstituted 2,3,6,7-tetraalkoxy-9,10-dihydroanthracenes, including six stereoisomeric pairs, were studied. The loss of the 9-(or 10-)substituent constitutes the main fragmentation route. The resulting ions give rise to a remarkable violation of the so-called even-electron rule as they eject the second substituent at the 10-(or 9-)position as a radical. The latter aspect was particularly investigated with the aid of low-energy (nominal value 15 eV) and mass-analysed ion kinetic energy spectra

Consolidation of waterlogged woods by in-situ polymerisation of isoeugenol

Variazione della componente aromatica di mele “Pink Lady” in risposta al vigore del portinnesto e agli apporti idrici.

RIDUZIONE DEL CONTENUTO DI MICOTOSSINE DOPO TRATTAMENTO CON RADIAZIONI IONIZZANTI VALUTATO SU CAMPIONI DI MANDORLE SICILIANE

Influence of wine-making technique and aging on the volatile fraction of red wine cultivars Perricone by SPME-GC/MS. In: Book of abstracts