Iron Fortification of Milk and Dairy Products

Blood haemoglobin level is used to define anaemia. Thresholds are given by category of age, of sex, and also of physiological status such as pregnancy. For example, the threshold is 120 g L–1 of haemoglobin in blood for a nonpregnant adult woman, 110 for a pregnant woman, 130 for a man older than 15 years, 110 for a child under 5 years… Iron deficiency constitutes the most important worldwide problem of human nutrition. In 2005, 1.62 billions of people were concerned by iron deficiency. Iron deficiency is encountered mostly in poor countries and particularly in Western Pacific, except Australia and New Zealand, in India and Pakistan, in the great majority of countries in Africa, in South Am…

Impact of Destroying the Structure of Model Gels on Volatile Release

International audience; The release of a strawberry aroma from different composite gels taken as models of fruit preparations and from a sucrose solution was investigated. The composition of the model systems differed with regard to the gelling agent, either pectin or carrageenan, and to the rigidity of the gel. With the use of atmospheric pressure chemical ionization−mass spectrometry, the release profiles of the aroma compounds were determined under stirring. At the same time, purge and trap measurements were performed to determine the release profiles of the aroma compounds without stirring. The comparison of the patterns obtained using these two complementary methods made it possible to…

Identification of Volatile Compounds in Blackcurrant Berries: Differences Among Cultivars

Berries of blackcurrant are known to produce a strong flavor. Some previous studies have reported that a given cultivar of blackcurrant can produce berries with a specific profile of volatile compounds. For the Burgundy region in France, the Noir de Bourgogne cultivar is especially important because it is the main ingredient of a liquor with a designation of origin. The aim of the present study was to characterize the volatile fractions of berries from 15 cultivars in order to explore the possibility of using different cultivars for liquor production. The plants were cultivated under the same conditions and harvested in the same year. The volatile fractions of the harvested berries were ana…

Food Flavors

International audience

Effect of konjac glucomannan addition on aroma release in gels containing potato starch.

International audience; The present study aimed to measure the retention of aroma compounds (ethyl acetate, ethyl hexanoate and carvacrol) in dispersions based on konjac glucomannan and/or potato starch, and to highlight the influence of konjac glucomannan on the mechanisms involved in aroma retention. Publications on the effect of konjac glucomannan on aroma release are scarce. Konjac glocomannan is a polysaccharide used as a food additive for its viscous and emulsifying properties. Retention of aroma compounds in dispersions was calculated from partition coefficients which were measured using the phase ratio variation method. This method, consisting of analyses of the headspace at equilib…

L'arôme du cassis

Feasibility and application of solvent assisted flavour evaporation and standard addition method to quantify the aroma compounds in flavoured baked matrices

International audience; In order to determine the feasibility of the solvent assisted flavour evaporation extraction (SAFE) technique and the standard addition method to quantify the aroma compounds retained in baked matrices after processing, three doughs that mainly vary in one ingredient, M1 (with eggs and palm oil), M2 (without eggs but with palm oil) and M3 (without eggs and without palm oil) were flavoured with an aromatic formulation of 19 different aroma compounds in propylene glycol that is responsible for a viennoiserie aromatic note. From the 19 aroma compounds added, 12 of them were quantified, so was also the propylene glycol. After the application of standard addition method 3…

Chapitre 11: Goût des ingrédients et des produits cosmétiques

International audience

Impact of oxidoreduction potential and of gas bubbling on rheological properties of non-fat yoghurt

International audience; The aim of this work was to study the effect of different gaseous conditions on the physico-chemical properties of yoghurt. Four conditions of oxidoreduction potential (Eh7), +433 mV (milk gassed with air), +405 mV (ungassed milk), +283 mV (milk gassed with N2) and −349 mV (milk gassed with N2–H2) were applied to milk. The rheological properties and microstructure of these yoghurts were determined by measuring apparent viscosity, whey separation (WS) and using confocal laser scanning microscopy. Exopolysaccharide (EPS) production by lactic acid bacteria (LAB) was also studied, and production was increased for extreme Eh7 values: the highest values were obtained for a…

The effect of salt content on the structure of iota-carrageenan systems: Na-23 DQF NMR and rheological studies

International audience; 23Na NMR spectroscopy has been used to study the effects of Na+ ion concentrations on the structure of 1% (w/w) iota-carrageenan systems, a natural gelling polysaccharide used as a thickener in the food industry. Rheological and 23Na T1 relaxation time measurements revealed that gel formation correlates with decreases in ion mobility over the range of 0-3% (w/w) sodium content. 23Na single-quantum (SQ) and double-quantum-filtered (DQF) NMR experiments performed on these systems provided evidence for a bound sodium ion fraction in a specifically ordered environment. These results have allowed us to propose a model for the carrageenan gelation mechanism in the presence…

Analytical comparison and sensory representativity of SAFE, SPME, and Purge and Trap extracts of volatile compounds from pea flour

Pisum sativum is of great economic and nutritional interest due to its protein content. Nevertheless, pea products are underused as a protein source in human food because of their strong beany flavour. Therefore, the objective of this study was to select an efficient and representative method to extract volatile molecules of pea flour. In the first step, three extraction methods were chosen: solid phase micro extraction (SPME); Purge and Trap extraction and solvent assisted flavour evaporation (SAFE). The corresponding extracts were analysed by gas chromatography coupled with mass spectrometry. In the second step, the sensory representativity of the extracts was assessed either by direct ga…

Effect of high hydrostatic pressure on extraction of B-phycoerythrin from Porphyridium cruentum: Use of confocal microscopy and image processing

International audience; The aim of the study was to extract B-phycoerythrin from Porphyridium cruentum while preserving its structure. The high hydrostatic pressure treatments were chosen as extraction technology. Different methods have been used to observe the effects of the treatment: spectrophotometry and confocal laser scanning microscopy followed by image processing analysis. Image processing led to the generation of masks used for the identification of three clusters: intra, extra and intercellular. All methods showed that high hydrostatic pressure treatments between 50 and 500 MPa failed to extract B-phycoerythrin from Porphyridium cruentum cells. The fluorescence emission was negati…

Impact of the structure of polysaccharide-based food gels on the release of aroma compounds: an APCI-MS approach

International audience

Effect of oxidoreduction potential on aroma biosynthesis by lactic acid bacteria in nonfat yogurt

 ; The aim of this study was to investigate the effect of oxidoreduction potential (Eh) on the biosynthesis of aroma compounds by lactic acid bacteria in non-fat yogurt. The study was done with yogurts fermented by Lactobacillus bulgaricus and Streptococcus thermophilus. The Eh was modified by the application of different gaseous conditions (air, nitrogen, and nitrogen/hydrogen). Acetaldehyde, dimethyl sulfide, diacetyl, and pentane-2,3-dione, as the major endogenous odorant compounds of yogurt, were chosen as tracers for the biosynthesis of aroma compounds by lactic acid bacteria. Oxidative conditions favored the production of acetaldehyde, dimethyl sulfide, and diketones (diacetyl and pen…

Rheological Properties and Flavor Release

International audience; Food texture may be of concern at different levels but is a main concern during food consumption. In fact, the way to handle food depends a lot on its texture: beyond interindividual differences, liquid products are swallowed more rapidly than solid products. e strength applied by teeth to bite food products and to transform the food bolus depends a lot on food stiffness. When food is eaten, it undergoes many modifications due to mastication, but also dilution through the addition of saliva, and variation in temperature. ese modifications result in great changes in food structure and texture, and thus involve significant changes in the partition of aroma compounds be…

Vapour partition of aroma compounds in strawberry flavoured custard cream and effect of fat content

International audience; Gas–matrix partition coefficients (k) of volatile compounds of a strawberry flavour added to a complex model system (custard dessert, COST action 921 recipe) were determined. The influence of fat content on the volatility of the strawberry aroma compounds was investigated using full fat milk- (FFM) and skimmed milk- (SM) custards. For the purpose of this study, the phase ratio variation (PRV) method was used because of its easy application for a mixture of volatiles without requiring any calibration. From the 15 aroma compounds present in the flavouring mixture, seven were volatile enough to be detected and concentrated enough to allow the calculation of k values. Ga…

Influence of Physicochemical Interactions between Amylose and Aroma Compounds on the Retention of Aroma in Food-like Matrices

In food matrices, where starch is often used as a gelling or texturing agent, the occurrence of amylose-aroma complexes and their effect on the release of aroma compounds are difficult to determine. Indeed, thick or gelled systems are known to reduce the diffusion rate of flavor molecules, resulting in an increase of retention. Moreover, interactions between aroma compounds and matrix components might increase the retention of aroma compounds. The complexing behavior of three aroma compounds with amylose was studied by DSC and X-ray diffraction to determine the relative importance of these two factors. Their interaction properties were different: two of them formed complexes, and the third …

Influence of the composition and the structure of fruit preparations on the aroma release and diffusion

Effect of oxidoreduction potential and of gas bubbling on rheological properties and microstructure of acid skim milk gels acidified with glucono-delta-lactone

Milk oxidoreduction potential was modified using gases during the production of a model dairy product and its effect on gel setting was studied. Acidification by glucono-delta-lactone was used to examine the physicochemistry of gelation and to avoid variations due to microorganisms sensitive to oxidoreduction potential. Four conditions of oxidoreduction potential were applied to milk: milk was gassed with air, nongassed, gassed with N(2), or gassed with N(2)H(2). The rheological properties and microstructure of these gels were determined using viscoelasticimetry, measurement of whey separation, and confocal laser scanning microscopy. It appeared that a reducing environment led to less-aggre…

Characterisation of odour active compounds along extraction process from pea flour to pea protein extract

International audience; Pisum sativum, rich in proteins, represents a main interest for human food. Nevertheless, pea products are underused because of their organoleptic characteristics. The extraction process of the proteins can partly explain the development of the typical flavour.The objective of the present study was to identify odour active compounds and to follow their evolution during four steps of the process (from pea flour to pea protein).Firstly, volatile compounds were extracted by Solvent Assisted Flavour Evaporation from each step and analysed by Gas Chromatography coupled with Mass Spectrometry and Olfactometry. Secondly, the volatile compounds, identified as odour active in…

The Potential Use of Raw and Deodorized Non-Conventional Protein Powder in Human Food

This study aimed to evaluate the potential use of a non-conventional protein source, a powder made from earthworms, as human food. Sensory analyses were done in order to assess the acceptability of cookies that were fortified with Eisenia foetida (Ef) protein powder. Cocoa and cinnamon were used as taste-masking agents. The acceptability of cookies fortified with deodorized Ef protein powder (off-flavor reduced) was also evaluated. We demonstrated that cocoa cookies can be fortified with 3.9% of raw Ef proteins and that cocoa/cinnamon cookies can be fortified with 5.2% of raw Ef proteins without any loss in their acceptability. Cookies fortified either with a raw protein powder or with a de…

Impact of Ageing on Pea Protein Volatile Compounds and Correlation with Odor

Vegetal proteins are of high interest for their many positive aspects, but their ‘beany’ off-flavor is still limiting the consumer’s acceptance. The aim of this work was to investigate the conservation of pea protein isolate (PPI) during time and especially the evolution of their organoleptic quality under two storage conditions. The evolution of the volatile compounds, the odor and the color of a PPI has been investigated during one year of storage. PPI was exposed to two treatments mimicking a lack of control of storage conditions: treatment A with light exposition at ambient temperature (A—Light 20 °C) and treatment B in the dark but with a higher temperature …

Effect of Microbial strains on Hexanal

The positive effects of plant-based protein consumption have been reported, nonetheless, several disadvantages suchgrassy or beany off-flavors are encountered (1) and are limiting the consumers acceptance towards these proteins.In this project, we investigated the potential of selected microorganisms to metabolize compounds responsible forthe leguminous off-flavor. Hexanal, well-known as a main contributor to the beany descriptor (2), was chosen for thisstudy.A selection of 16 strains representative of different fermentative types has been used to treat a liquid model matrixcontaining hexanal at 2 mg/L. The strains were applied on the medium at the end of their growing phase. Theinoculum wa…

Point sur le projet "Pérennité et développement de la filière cassis en Bourgogne"

L’association interprofessionnelle des Acteurs du Cassis en Bourgogne, qui a vu le jour en 2016, est le porteur du projet « Pérennité et développement de la filière cassis en Bourgogne ». Démarré en 2018, celui-ci rassemble plusieurs partenaires techniques et scientifiques qui travaillent conjointement pour trouver des solutions aux difficultés rencontrées par la filière face aux aléas climatiques et sanitaires. Ainsi, le CNRS, SAYENS, les laboratoires SPIRAL, AgroSup Dijon et la Chambre d’Agriculture de Côte-d’Or, constituent le groupe opérationnel qui travaille sur les 3 axes identifiés comme cruciaux pour stabiliser les rendements du cassis : le développement de mesures agro-écologiques …

Influence of complexation between amylose and a flavored model sponge cake on the degree of aroma compound release

International audience; Flavoring is used in the food industry to reinforce the aroma profile of baked cereal goods. During the processing of such products, interactions between starch and aroma compounds can occur, and this may have an impact on aroma release and perception. In the present study, 20 aroma compounds were tested to establish whether they formed complexes with amylose. The structure of the complexes was determined by wide-angle X-ray scattering (WAXS). A cocomplexation study proved that several complexing compounds could be present in the same crystalline aggregate. WAXS and differential scanning calorimetry (DSC) experiments were performed in a flavored model sponge cake at …

The Off-Flavor of Pea Flour

Pea products are underused as a protein source in human food because of their “beany” flavor. The objective of the study is to select an extraction method being the most representative for the sensory perception of the beany flavor. Solid phase microextraction (SPME) and purge and trap (P&T) extracts were analyzed by GC-MS and the sensory representation of the extracts was assessed by direct gas chromatography-olfactometry (D-GC-O). Extracted compounds were less numerous in SPME extracts than in P&T extracts. Nevertheless, the SPME method was more suitable because of its good representation of the pea suspension odor.

Release of isoamyl acetate from starch pastes of various structures : thermodynamic and kinetic parameters

The release of isoamyl acetate from starch-based matrices was studied on the basis of a cumulative dynamic headspace analysis. Two corn starches were compared, which are known to yield pastes and gels differing in their structures and properties. These properties were assessed by viscometry and viscoelastic measurements. Aroma release was discussed as a function of the structure and texture parameters of the matrix. The release curves obtained from water and from the various starch-based matrices at 25 degrees C showed similar patterns but differed in their initial slopes and in the final plateau values. The lowest initial slopes were obtained for the normal starch dispersions that formed g…

Allergies et intolérances alimentaires : Fiches pratiques sur les 14 allergènes majeurs

International audience; Les allergies et les intolérances alimentaires représentent un enjeu majeur pour l’industrie agroalimentaire. Présenté sous forme de fiches pratiques, cet ouvrage a pour objectif d’apporter une vision claire et synthétique sur le sujet. Il présente notamment les 14 allergènes majeurs et détaille pour chacun d’eux : la nature et les causes de l’allergie ; les préparations courantes dans lesquelles on le trouve, ses rôles nutritif et technologique ; les moyens pour le remplacer, selon que l’on souhaite conserver le goût, les valeurs nutritives ou simplement l’apparence.Les questions d’hygiène et de la maîtrise du risque sont également abordées, et une démarche pratique…

Potential of microorganisms to decrease the "beany" off-flavor

Partition of volatile compounds in pea globulin–maltodextrin aqueous two-phase system

International audience; This study is based on the assumption that the off-flavour of pea proteins might be decreased using the retention of volatile compounds by a mixture with another biopolymer. The partition of volatile compounds in an aqueous system containing pea protein and maltodextrins was followed under thermodynamic incompatibility conditions. Firstly, the phase diagram of the system was established. Then, the partition of aroma compounds between the phase rich in protein and the phase rich in maltodextrin was measured by SPME–GC–MS. There was a transfer of volatile compounds during phase separation. Variations of pH were also used to vary the retention of volatile compounds by p…

Chapitre 15: le goût des ingrédients et des produits cosmétiques

International audience

Flavor release from complex food systems

International audience

Flavors and their interaction with food structure

International audience

Physico-chemical interactions between starch and aroma compounds and their effects on aroma retention in food like matrices

International audience

Retention of aroma compounds in starch matrices: competitions between aroma compounds toward amylose and amylopectin

International audience; The retention of three aroma compounds-isoamyl acetate, ethyl hexanoate, and linalool--from starch-containing model food matrices was measured by headspace analysis, under equilibrium conditions. We studied systems containing standard or waxy corn starch with one or two aroma compounds. The three studied aroma compounds interact differently: ethyl hexanoate and linalool form complexes with amylose, and isoamyl acetate cannot. However, in systems containing one aroma compound, we observed with both starches a significant retention of the three molecules. These results indicate that amylopectin could play a role in the retention of aroma. In systems containing two arom…

Measurement of transport phenomena of volatile compounds: A review

International audience; Measurement of the transport phenomena of volatile compounds is a central preoccupation in food flavour and is the subject of the present paper. After a theoretical reminder concerning transport phenomena, the methods used for the measurement of diffusion and mass transfer of volatile compounds are described with their principles, main advantages and drawbacks. These methods are illustrated by some results obtained for flavoured model food products.Several methods characterized by the type of diffusion coefficient measured, the method basis, the scale at which diffusion is measured and the means used to detect the diffusing molecule, are available to determine diffus…

Substitution of carcinogenic solvent dichloromethane for the extraction of volatile compounds in a fat-free model food system

International audience; Dichloromethane is known as a very efficient solvent, but, as other halogenated solvents, is recognized as a hazardous product (CMR substance). The objective of the present work is to propose substitution solvent for the extraction of volatile compounds. The most important physico-chemical parameters in the choice of an appropriate extraction solvent of volatile compounds are reviewed. Various solvents are selected on this basis and on their hazard characteristics. The selected solvents, safer than dichloromethane, are compared using the extraction efficiency of volatile compounds from a model food product able to interact with volatile compounds. Volatile compounds …

Retention of Carvacrol and Ethyl Acetate in Aqueous Gels of Potato Starch and Konjac Glucomannan

The partition coefficients of two volatile compounds in dispersions containing potato starch (PS) and/or konjac glucomannan (KGM) have been determined by the phase ratio variation method using equilibrium headspace analysis by gas chromatography. The influence of the moment when volatile compounds were added and the effect of the polysaccharide dispersions were investigated. For ethyl acetate, retention by KGM was based on viscosity effect. Retention of carvacrol was important when amylose was accessible. The addition of KGM to PS suspensions decreased the retention governed by interactions with starch.

Flavour release from poly saccharide gels : different approaches for the determination of kinetic parameters

International audience; Two different approaches were developed to quantify aroma release: static headspace syringe injection analysis at different times, and dynamic headspace analysis with trapping on Tenax® for different times. They were applied on two polysaccharide matrices in order to better understand relationships between the structure of the food matrix and flavour perception. These two approaches allowed the determination of kinetic and thermodynamic parameters. Dynamic methods better simulate flavour release in the mouth, but only static measurements allow a determination of thermodynamic and kinetic parameters with a good precision.

Physico-chemical characterisation of a non-conventional food protein source from earthworms and sensory impact in arepas

International audience; This study aimed to characterise a non-conventional protein source: a powder made from earthworms, and to evaluate its potential use as human food. The way it was prepared led to low solubility and wide particle size distribution. Sensory analysis was used to assess the acceptability and the organoleptic properties of maize-based pan-cakes fortified with this novel protein powder. Satisfying products were obtained with a substitution level of 5.5% (w/w) earthworm powder in pan-cakes. GC-MS allowed the identification of more than seventy volatile compounds that may be responsible for the off-odour of the powder. The most abundant chemical groups found in the volatile …

Impact of lipid extraction on the dearomatisation of an Eisenia foetida protein powder

www.elsevier.com/locate/foodchem ; FSTA:2010-Sp0197; International audience; Delipidation was studied as a way to dearomatise a non-conventional protein powder obtained from Eisenia foetida earthworms. In the first step, we studied the impact of several factors such as solvent type, extraction method and particle size on the yield of extracted lipids. Lipid extraction from samples was considerably improved using an ultrasound method with a chloroform/methanol mixture and small particle size. In the second step, the volatile compounds were extracted from the delipidated protein powder by the SAFE extraction method and by the HS-SPME method, and were further analysed by GC-MS. The chloroform/…

Chapter 7. Physico-chemical properties of konjac glucomannan

International audience; The konjac flour is obtained from the tuber of the Amorphophallus konjac, a plant of the Araceae family. This plant is found in the wild and cultivated as a vegetable in Thailand, China, Vietnam, Korea, and Japan since the nineteenth century AD (Chua et al. 2012; Li et al. 2005). The major compound of konjac flours is glucomannan, the reserve polysaccharide of konjac. The glucomannans are also present under the same structural type in various plant species: orchid bulbs, lily, and Aloe vera seeds (Dorthe 2005). The konjac flour is obtained by crunching the tubers more or less finely. These are harvested after 2 or 3 years of plant development. The tubers are cut, dri…

Chapter 6: Iron fortification of milk and dairy products

International audience; Anaemia is a worldwide health problem, mainly due to the low availability of iron in food products. The bioavailability of heme iron is far greater than the one of non-heme iron. Dairy products consumed together with iron-rich food decrease the availability of iron but the competition between calcium and iron ions is not clear. Iron is naturally present in milk under the form of lactoferrin, but lactoferrin may undergo thermal denaturation during process. Iron salts, cheaper than lactoferrin, are preferably used to fortify dairy products. The bioavailability of iron depends on the solubility and the dissociation constant of the salts. To administer iron as a medicine…

Instrumental and sensory evaluation of the retention of aroma compounds, alone or in blends, by starchy products

National audience

Impact of oxydoreduction potential on biosynthesis of volatile compounds in acid skim milk gel: identification and quantification

International audience; Oxydoreduction potential (Eh) is an environmental parameter which defines balances between oxidant species (acceptors of electrons) and reduced species (donors of electrons) of a medium. It may modify the growth capacity of microorganisms, the metabolic fluxes, participate to the quality of fermented products (2, 6, 7) and affect the production and/or stability of volatile compounds (3,5). (...)

Rôle des différents constituants d'un aliment dans le comportement des composés d'arôme

National audience