Probiotics Properties: A Focus on Pregnancy Outcomes.

A healthy microbiome plays an important role in the prevention of illness and maintenance of overall health, including reproductive health. Although the therapeutic advantages of probiotics have been shown to run across multiple organ systems, their role in pregnancy is not well explored. The aim of this review is to highlight the potential advantages and adverse effects of probiotics in pregnancy. Data were collected from the literature over the past decade using PubMed, Medline, Google Scholar, Ovid, Scopus, and Science Direct. A total of 40 articles were utilized in this review. Collected data indicated that prenatal and post-natal supplementation with lactobacilli alone or lactobacilli …

Expression and localization of MMP-9, MMP-2 and iNOS in some sample of human dental pulp orthodonticaly treated.

Hsp27 and Hsp60 in human submandibular salivary gland: Quantitative patterns in healthy and cancerous tissues with potential implications for differential diagnosis and carcinogenesis

Tumors of the submandibular salivary gland (SMG) are uncommon but sufficiently frequent for the physician to consider them in routine examinations and for the pathologist to be prepared to differentiate them from other tissue abnormalities. However, scarcity of specimens makes training difficult, a situation compounded by the lack of accepted universal diagnostic guidelines. Furthermore, there is little information on the chaperone system (CS) of the gland, despite the increasing evidence of its participation in carcinogenesis as a biomarker for diagnosis and patient follow up, and in the mechanisms by which the tumor cells thrive. We are investigating this aspect of various tumors, and her…

Immunohistochemical and molecular expression of CB1 receptors in human colonic segment. Preliminary results

Recent studies document that CB1 cannabinoid receptors, considered since its first identification as “brain specific”, could be expressed by peripheral tissues targets (adipose organs, enteric nervous system, striated muscle, epatocytes). This receptors were found in the GI tract of different species, including mice, rats, guinea pigs, pigs and humans. Endocannabinoids play a role in the pathophysiology of the gastrointestinal tract. They play a role in emesis, acid-related disorders, motility-related disorders, irritable bowel syndrome and diarrhea, and in inflammatory bowel disease. Endocannabinoid system might be involved in cancer differentiation, growth and cell migration. Distribution…

CAPSAICIN STIMULATION AND NEUROGENIC EXPERIMENTALLY INDUCED INFLAMMATION OF THE HUMAN LABILA MUCOSA

Expression of Cyclooxygenase-1 and Cyclooxygenase-2 in normal and pathological human oral mucosa

Abstract: Cyclooxigenase (COX) is the rate-limiting enzyme for the conversion of arachidonic acid (AA) to prostaglandins (PGs). Two isoforms of COX have been identified: COX-1 is constitutively expressed in many cells and is involved in cell homeostasis, angiogenesis and cell-cell signalling; COX-2 is not expressed in normal condition however it is strongly expressed in inflammation. The oral cavity is costantly exposed to physical and chemical trauma that could lead to mucosal reactions such as hyperplasia, dysplasia and cancer. Early diagnosis is the most important issue to address for a positive outcome of oral cancer; therefore it would be useful to identify molecular markers whose expr…

The natriuretic system in human and murine submandibular salivary glands in health and disease: insights from head and neck tumour cases

Introduction: The wide distribution of the atrial natriuretic peptide was paralleled by a similar presence of its receptors, an indication of the wide range of their biological effects beyond the control of the homeostatic process. This study depicts through immunohistochemical and molecular techniques the distribution and modulation of atrial natriuretic peptide and its receptors in human and murine salivary glands. It also highlights some clinical implications of ANP dysregulation. Methods: Human SMG’s tissue samples were biopsied from normal (3), people with benign (2) or with malignant tumors (8). In addition, biopsies were taken from normal mice (10). They were processed by standard me…

Expression of beta endorphin and caspase 9 in orthodontically treated dental pulp

Cordon ombilical. Nouvelles sur l’acétylcholine

Introduction L’acetylcholine (Ach) fut la premiere substance impliquee dans la transmission synaptique. Elle est synthetisee a partir de l’enzyme acetyl-choline transferase (ChAT) et est degradee par l’acetyl-choline esterase (AchE). Il existe des anticorps specifiques diriges contre la ChAT et l’AchE qui ont permis de mettre en evidence la distribution de ce neurotransmetteur au niveau des systemes nerveux central et peripherique. Il faut rappeler que les neurones somato-moteurs, pre-ganglionnaires du tronc cerebral et de la moelle epiniere, les neurones post-ganglionnaires visceraux des ganglions parasympathiques et de quelques ganglions orthosympathiques sont cholinergiques. Ces donnees …

Immunohistochemical expression of Cytokeratins in periapical lesions

Objective. This study seeks to clarify the histological origin of apical lesions, through the expression of certain cytokeratins.Study design. In 3 years 30 patients were selected. After clinical and radiological exams were chosen patients who had severe apical lesions and tooth decay with exposure of the pulp chamber left untreated for very long periods (more the 12 months). Serial slides were prepared both for immunohistochemistry and Hematoxylin- Eosin. Material and methods. Twenty specimens coming from the 30 patients were used for our purpose. All periapical lesions were surgically extracted and fixed in Bouin mixture, and embedded in paraffin. Samples were processed immunohistochemica…

INFLAMMATORY BOWEL DISEASE AND COLORECTAL CANCER, NUTRACEUTICAL ASPECTS

Nutraceuticals constitute a group of functional foods that provide added health benefits for various disorders including inflammatory bowel disease (IBD) and colorectal cancer (CCR). The main groups of nutraceuticals include probiotics, prebiotics omega 3 and antioxidants. Studies on nutraceutical showed that this type of food possessed similar properties to drugs but with the benefit of not having side effects. This mini review shows that probiotics and prebiotics, when administered simultaneously with traditional therapies, reduce IBD symptoms and reduce synthesis of enzymes probably involved in colorectal carcinogenesis. Moreover, Omega 3 reduces the synthesis of inflammation mediators a…

Role of Matrix Metalloproteinases 2,9 (MMP-2,-9), Orphanin FQ and Others Peptides in Vascular Tone of Human Umbilical Cord at Term

Umbilical cord is the structure that connects the fetus to the placenta. Umbilical cord at term is made of three vessels surrounded by the Wharton's Jelly, a mocous connective tissue rich in glycosaminoglycans, proteoglycans and different types of collagens and other microfibrils, which are responsible for its elasticity and its mechanical properties. Umbilical cord seems to lack any capillaries or lymphatics and umbilical vessels are unique in lacking any innervation of the vascular tone, and the expression of some enzymes responsible for the remodelling of the extracellular matrix. Trough immunihistochemistry we investigated the expression of matrix metallo-peptidase 2 and 9 (MMP-2, MMP-9…

Penphigus Vulgaris in a mediterranean Young Adult Manifesting as Desquamative Gingivitis

Molecular chaperones in tumors of salivary glands.

The salivary glands are key components of the mouth and play a central role in its physiology. Their importance may be appreciated considering their number, occurrence in pairs, and distribution in the mouth: two parotids, two submandibular, two sublingual, and many other small ones scattered throughout the mouth. They produce saliva, without which ingestion of non-liquid nutrients and speech would be practically impossible. Nevertheless, the physiology and pathology of salivary glands are poorly understood. For instance, tumors of salivary glands occur, and their incidence is on the rise, but their etiology and pathogenesis are virtually unknown, although some risk factors have been identi…

Immunohistochemical expression and apoptotic role of Caspase9 in aortic aneurysms

Role of K-RAS and P53 Gene in Salivary Glands Tumorigenesis

MMP-2, MMP-9, and iNOS Expression in Human Dental Pulp Subjected to Orthodontic Traction

Abstract Objective: To test the hypothesis that some metalloproteinases (MMP-2, MMP-9) and inducible nitric oxide synthetase (iNOS) enzymes in dental pulp samples do not vary when subjected to orthodontic treatment. Materials and Methods: Human dental pulps were taken from male and female patients (N=10; age 10–14 years). A straight wire technique was used with nickel-titanium or steel archwires. The increase of pressure applied on teeth was gradual. Five patients were subjected to premolar extractions after 14 months of treatment and one after 24 months. Samples were Bouin-fixed, paraffin-embedded, and afterwards processed for immunohistochemistry using anti-MMP-2, anti-MMP-9, and anti-iNO…

Immunohistochemical Localization of Epithelial, Endothelial and Muscular Cell Differentiation Markers in Human Umbilical Cord

Introduction. Since time [1], our group studies through immunohistochemistry and RT-PCR analysis the expression of many genes and the localization of the corresponding proteins in the human umbilical cord [2], with the aim to create a map of the presence of the different molecules in the different territories of this organ. In this work we investigate the expression and localization of several cell differentiation markers in the different territories of the human umbilical cord. Materials and methods. The presence of E-Cadherin, CD31, CD68, AE1-AE3, Desmin, Actin ML, HHF35 and MIB-1 was studied through immunohistochemistry. Results. The expression of E-Cadherin (epithelial cell marker) has …

Oral manifestations of inflammatory bowel disease

Inflammatory bowel diseases (IBD), including Crohn’s disease and ulcerative colitis, have important extraintestinal manifestations, notably in the oral cavity. These oral manifestations can constitute important clinical clues in the diagnosis and management of IBD, and include changes at the immune and bacterial levels. Aphthous ulcers, pyostomatitis vegetans, cobblestoning and gingivitis are important oral findings frequently observed in IBD patients. Their presentations vary considerably and might be well diagnosed and distinguished from other oral lesions. Infections, drug side effects, deficiencies in some nutrients and many other diseases involved with oral manifestations should also b…

NUTRACEUTICALS IMPROVE EXPERIMENTAL COLITIS: PLACE OF CATECHINS IN THE 2,4,6-TRINITROBENZENE SULFONIC ACID MODEL RATS

Background: Nutraceuticals provide added health benefits for inflammatory bowel disese (IBD) and Epigallocatechin-3-galate (EGCG), a green tea catechin, has been shown to possess such anti-inflammatory and anti-oxidant effects. Aim: To evaluate the molecular modulation of Reactive Oxygen Species (ROS), Tumor Necrosis Factor-alpha (TNF_alpha), nuclear factor Kappa Beta (NF-Kbeta), Interleukin 6 (IL-6) by EGCG on experimental colitis. Matherial and Methods: Thirthy five male sprague-dawley rats were randomly divided into 4 groups: Normal control group (n=5), EGCG group (n=9), TNBS group (n=9), and TNBS + EGCG group (n=12). For both TNBS and EGCG treated groups, 1 mg/Kg EGCG was administered d…

Multiple Supernumerary Teeth with no Other Associated Diseases or Syndromes:Case Report

Cytokeratins identification in human Umbilical Cord at Term

NUTRITION, OXIDATIVE STRESS AND INTESTINAL DYSBIOSIS: INFLUENCE OF DIET ON GUT MICROBIOTA IN INFLAMMATORY BOWEL DISEASES.

Background: Microbiota refers to the population of microorganisms (bacteria, viruses and fungi) that inhabit the entire gastrointestinal tract, more particularly the colon whose role is to maintain the integrity of the intestinal mucosa and control the proliferation of pathogenic bacteria. Alteration in the composition of the gut microbiota is called dysbiosis. Dysbiosis redisposes to inflammatory bowel diseases such as ulcerative colitis, Crohn disease and indeterminate colitis. Methods: The purpose of this literature review is to elucidate the influence of diet on the composition of the gastrointestinal microbiota in the healthy gut and the role of diet in the development of dysbiosis. Co…

Localisation immunohistochimique du facteur natriurique atriale du tissu muqueux mûr du Cordon Ombilical humain après naissance et dans la Pulpe Dentaire

Modulation of MMP-2 and MMP-9 in Churg-Strauss syndrome respiratory mucosa: potential monitoring parameters.

Churg-Strauss (CSS) syndrome is rare and of unknown etiology. It is associated with vasculitis, blood eosinophilia and granulomatosis, and affects multiple organs and systems at various stages of the disease. Specific diagnostic and monitoring tests are not yet available. This study aims to assess the changes in MMP-2 and MMP-9 along with the histopathological alterations in two cases of CSS, as possible potential diagnostic and monitoring criteria. Two adult male patients were diagnosed with CSS in the otorhinolaryngology clinic in the University of Palermo, based on multiple clinical and histopathologic criteria. Biopsies of respiratory mucosa were taken after the consent of the patients…

CAPSAICIN STIMULATION AND NEUROGENIC EXPERIMENTALLY INDUCED INFLAMMATION OF THE HUMAN LABIAL MUCOSA

Background. The neurogenic inflammation depends on the neuromodulator release by peripheral nervous termi−nations. A series of different studies proved the existence of a neurogenic phlogosis (NP) in different body sites.Only experiences on rat managed to induce NP at the oral mucosa level.Objectives. Inducing NP at the human mucosa level.Material and Methods. Two groups of patients (group A: M/F: 8/7; mean: 41.14 ± 12.4; range: 24–60; group B:M/F: 8/7; mean: 41.21 ± 12.39; range: 23–60) were examined in authors’ laboratory. The NP was experimentallyinduced at the lower lip mucosa level after topically applying capsaicin. The vascular consequences were observedthrough computer videocapillar…

LE REMANIEMENT DE LA MATRICE METALLO-PROTEINASES EST IMPLIQUEE DANS L'ODONTOGENESE? ETUDE DU MMP-9

HGF-Induced Branching in MDCK Cells is Dependent on the Activation of MEK1 and the Inhibition of GSK-3

CANALAR REPROCESSING WITH NI-TI INSTRUMENTS: OUR EXPERIENCE.

Hsp60 in Inflammatory Disorders

Heat shock proteins (HSP) including HSP60 are immunogenic proteins shared by particular microbial agents and mammals. HSP60 has been implicated in multiple inflammatory disorders and autoimmune diseases mostly through its interactions with the immune system. Such diseases include inflammatory bowel disease, chronic obstructive pulmonary disease, Hashimoto’s thyroiditis, myasthenia gravis, multiple sclerosis and even atherosclerosis plaques among others. It is present in the cytosol, cell membrane, cell surface as well as in the extracellular space and in the circulation. As a super antigen, HSP60 has the dual role as an immunomodulator and as a biomarker, a node molecule in balance between …

Expression of Nitric Oxide Synthase in Inflamed Gingival Tissue: Immunoistochemical and Image Analysis

Fludarabine prevents smooth muscle proliferation in vitro and neointimal hyperplasia in vivo through specific inhibition of STAT-1 activation.

Drug-eluting stents are increasingly used to reduce in-stent restenosis and adverse cardiac events after percutaneous coronary interventions. However, the race for the ideal drug-eluting stent is still on, with special regard to the best stent-coating system and the most effective and less toxic drug. Fludarabine, a nucleoside analog, has both anti-inflammatory and antiproliferative cellular effects. The aim of the present study was to assess the cellular and molecular effects of fludarabine on vascular smooth muscle cell (VSMC) growth in vitro and in vivo and the feasibility and efficacy of a fludarabine-eluting stent. To study the biomolecular effects of fludarabine on VSMC proliferation…

Heat shock proteins in embryonic and adult submandibular salivary glands in healthy and tumorigenic tissues

CANALAR REPROCESSING G WITH NI-TI INSTRUMENTS: OUR EXPERIENCE.

Maxillary Bone Necrosis Following the Use of Formaldehyde-Containing Paste: Three Case Reports

Immunohistochemical expression of apoptotic factors, cytokeratins, and metalloproteinase-9 in periapical and epithelialized gingival lesions

Bellmann K, 2010, CELL STRESS CHAPERON, V15, P101, DOI 10.1007-s12192-009-0126-9; Cappello Francesco, 2011, Front Biosci (Schol Ed), V3, P341, DOI 10.2741-s155; Cappello F, 2006, CANCER, V107, P2417, DOI 10.1002-cncr.22265; Cappello F, 2002, EUR J HISTOCHEM, V46, P199; Carneiro E, 2009, ORAL SURG ORAL MED O, V107, P127, DOI 10.1016-j.tripleo.2008.07.030; Chandra D, 2007, J BIOL CHEM, V282, P31289, DOI 10.1074-jbc.M702777200; Fujita Y, 2011, ODONTOLOGY, V100, P215; Garcia Celia Carrillo, 2007, Med Oral Patol Oral Cir Bucal, V12, pE585; Garcia CC, 2009, ORAL SURG ORAL MED O, V107, pE43, DOI 10.1016-j.tripleo.2008.12.002; Gregory CD, 2011, J PATHOL, V223, P177, DOI 10.1002-path.2792; Gupta S, …

Comparative analysis of Hsp10 and Hsp90 in large bowel healthy mucosa and adenocarcinomas

Heat shock proteins (Hsps) are an important class of molecules with various functions. Their classic role is to assist other proteins in folding and re-folding and, when proteins are defective or irreversibly misfolded, to drive their degradation. For this reason, some Hsps are also named molecular chaperones. During evolution, this class of proteins has also acquired extrachaperoning roles such as participation in immune system regulation, cell differentiation, programmed cell death and carcinogenesis. Hsp10 is a partner of Hsp60 in the Hsp60/10 folding machine, but numerous scientific studies have shown that Hsp10 may also play other roles. In fact, Hsp10 seems to have an immunomodulatory…

IMMUNOLOGICAL ASPECTS OF CROHN'S DISEASE: A REGULATORY FUNCTION OF TIM-3/GALECTIN-9 IN LYTH1.

Crohn's disease (CD) is a type of inflammatory bowel disease (IBD) and its etiology is multifactorial and involves a combination of genetic environmental factors. The interaction of these factors causes an imbalance of the microbiota, leading to the activation of several immunological and inflammatory mechanism. From an immunological point of view, there seems to be an involvement of the TIM-3/GALECTIN-9 pathway and of the autoregulation of lyTh1. These studies show that in patients with CD the autoregulation of lyth1 is lost due to a reduced concentration of galectin-9 and a reduced TIM-3 expression in LyTh1. This could be one of the reason for the state of perpetual activation of lyTh1, r…

THE ROLE OF BUTYRIC ACID AS A OPROTECTIVE AGENT AGAINST INFLAMMATORY BOWEL DISEASE

Inflammatory Bowel disease (IBD), such as Crohn's disease and ulcerative colitis, are pathologies characterized by a chronic inflammation of the gastrointestinal tract. Their etiopathogenesis is not yet fully understood. Immune system and heat shock proteins (HSPs) dysfunctions are considered to be among the most likely causes of these diseases. Butyrate is a short-chain fatty acid produced by intestinal microflora. It has a trophic, benefical and protective role in the colonic mucosa, and it also induces changes in Hsp levels and localization. It may therefore be a valuable complementary therapeutic agent when used alongside trraditional drugs (mesalazine and corticosteroids) to treat the …

Localization of atrial natriuretic factor (ANF) in the normal pleura in rabbit. Immunohistochemical studies and PCR analysis

Bovine seminal vesicles ANP, Oxytocin, immunohistochemical expression.

Expression of β-Endorphin and Caspase-9 in Orthodontically Treated Dental Pulp.

Introduction. For several years, our histology laboratory has worked in co-operation with the Winchmore Hill Dental Practice studying the activity of Acetylcholinesterase and some neuropeptides (CGRP, Sub. P, Enkephalins, Endorphins)(1) in orthodontic treated human dental pulp to clarify force's action. It seems that recent data do not yet, fully elucidate the exact effects of the force applied on teeth. For this reason we are investigating whether the traction is involved in some cases of pulpal death. In this study we focused on the expression of Caspase-9 and β-Endorphine. Materials and Methods. The expression of the 2 peptides has been identified through immunohistochemistry assay: not …

A novel use of type 1 collagen gels in determinino the metastatic potential of tumor cells

Localization of Atrial Natriuretic Factor (ANF) in the Normal Parietal Pleura in Rabbit. Immunohistochemical Studies and PCR Analysis

Probiotics, prebiotics and symbiotics in inflammatory bowel diseases: state-of-the-art and new insights

Inflammatory bowel disease (IBD) consists of two distinct clinical forms, ulcerative colitis (UC) and Crohn's disease (CD), with unknown aetiology, which nevertheless are considered to share almost identical pathophysiological backgrounds. Up to date, a full coherent mechanistic explanation for IBD is still lacking, but people start to realize that the pathogenesis of IBD involves four fundamental components: the environment, gut microbiota, the immune system and the genome. As a consequence, IBD development might be due to an altered immune response and a disrupted mechanism of host tolerance to the non-pathogenic resident microbiota, leading to an elevated inflammatory response. Consideri…

Expression of MMP-2 and MMP-9 in odontogenic myxoma in a child: report of a clinical case

Odontogenic myxoma (OM) is a benign, locally invasive, non-metastasizing neoplasm of the jaw bones. Despite the benign nature of these lesions, there is a high rate of recurrence and the current recommended therapy, depending on the size and behaviour of the lesion, can vary from curettage with peripheral ostectomy, segmental resection up to radical resections for more aggressive lesions. OM is a rare tumour which occurs predominantly in the third decade of life and it is rare in children. Matrix metalloproteinases (MMPs) are a family of extracellular endopeptidases responsible for the degradation and remodelling of extracellular matrix, they are known to be involved in the progression and …

Does the orthodontic treatment induces dental pulp cellular death? Caspase-3 and-9, HSP60 and TUNEL expression

Objective: To evaluate the hypothesis of human dental pulp cell death during orthodontic treatement (O.T) and the degree of apoptosis through the expression level of the proteins Caspase-3,-9, TUNEL and HSP60. Materials and methods: Human dental Pulp were coming from both male and female patients (N=20; age 10-14years). The technique used was the Straight Wire, which involves Nickel-Titanium or Steel archwires. The increase of pressure applied on teeth was gradual. Some patients were subjected to a premolar extraction after 3 months treatment, and others after 6 months. Samples were Bouin-fixed, paraffin-embedded and afterwards processed for immunohistochemistry using anti-caspase-3,-9 anti…

EFFICACY OF ARGENTUM-QUARTZ SOLUTION IN THE TREATMENT OF PERIANAL FISTULAS: A PRELIMINARY STUDY

Objective: Nowadays, an optional and effective medical surgery remains the gold standard for perianal fistulas. Hereby we reported preliminary rsults in favor of using Argentum-Quartz solution for both primary and recurrent perianal fisrtulas. Methods: Three patients with intersphimncter and extrasphinteric fistulas were enrolled. Argentum-Quartz solution was administrated twice a week for sa period of 4 weeks, followed by a pause of 8 days and then another 4 weeks of treatment, totally 16 administrations. After treatment, all patients were monitored for 4-months follow-up. Results: Complete closures of 2 extrasphinteric fistulas and a partial closure with absence of inflammation and supera…

Intestinal microbiota mutualism and gastrointestinal diseases

The purpose of this work is to investigate the link between an altered intestinal mcro-biota or dysbiosis and chronic inflammatory disorders, in particular inflammatory bowel disease (IBD). Along with probiotics, faecal microbiota transplantation (FMT) opts to be a promising therapeutic treatment for restoring the bacterial homeostasis of the hu-man intestine and reducing the risk of colorectal carcinogenesis. Microbiota is the com-plex microbial flora that resides in the gut establishing a mutually beneficial relation-ship. Alteration of the microbiota’s composition, termed as dysbiosis, may lead to pathological conditions. Treatment with probiotics can restore the normal commensal flora i…

PERIODONTAL PANORAMIC CAPILLAROSCOPY: A NEW DIAGNOSTIC METHOD

Nandrolone decanoate interferes on testosterone biosynthesis and alters blood-testis barrier.

Nandrolone decanoate (ND) is a synthetic testosterone analogue considered one of the most commonly abused anabolic androgenic steroids by adolescents and athletes. ND is alleged to promote an increase in muscle mass and improves both physical appearance and sporting performance, but ND abuse is often associated with serious adverse effects, interfering with the endocrine system and the reproductive system. In a previous study, we demonstrated that ND treatment of Leydig cells interferes with the biosynthesis of testosterone in a dose increase-dependent fashion (1). As a consequence of the results obtained in vitro, in this study an animal model was utilized to better understand the side eff…

Superior Mesenteric Artery Syndrome: Clinical, Endoscopic, and Radiological Findings.

Background. The superior mesenteric artery (SMA) syndrome is a rare entity presenting with upper gastrointestinal tract obstruction and weight loss. Studies to determine the optimal methods of diagnosis and treatment are required. Aims and Methods. This study aims at analyzing the clinical presentation, diagnosis, and management of SMA syndrome. Ten cases of SMA syndrome out of 2074 esophagogastroduodenoscopies were suspected. A contrast-enhanced computed tomography (CECT) scan was performed to confirm the diagnosis. After, a gastroenterologist and a nutritionist personalized the therapy. Furthermore, we compared the demographical, clinical, endoscopic, and radiological parameters of these …

Expression of gelatinases and cycloxygenases in one case of myoepithelioma

Scanning Electronic Microscopy (SEM) Analysis of the ventriculo peritoneal shunts

Ventricular peritoneal derivations may determinate conditions in the tissues, which may then create fibrous tissues around them. They include cases with catheters of the SEM and they show that when the fibrous tissues are formed, the outer surface is irregular. However, they are not determined through phystolosis or irregular inside the surface

Expression of Nitric Oxide Synthase in inflamed gingival tissue: immunohistochemical and image analysis

We have used 12 Epulis taken during surgical operation and as control, some normal gingival specimen. The aim was to clarify the relationship between the degree of inflammation and NO production in several cases of Epulis, exhibiting different degree of inflammatory process and verifying the presence of cells involved in NO production. From the immuhistochemical data in our possession we can conclude that NO is one of the factor which module the pathogenecity degree of Epulis.

Nouvelle utilisation du Collagène de Type I dans la Détermination du potentiel metastatique des Cellules Tumorales : Etude Morphologique et Biochimique

Heat Shock Protein in embryonic and adult submandibular salivary glands in healthy and tumorigenic tissues

Heat Shock Proteins (HSPs) role in development and function of human salivary glands have not yet been measured or mapped in detail, in particular, HSP60 role is unknown. They can be potential biomarkers for glandular diseases and malignancies. We present our initial findings on HSP10, HSP27, HSP60, HSP70 and HSP90 distribution in human submandibular salivary glands (SMG) at various stages of development and tumorigenesis.

In-vitro regulation of odontogenic gene expression in human embryonic tooth cells and SHED cells

The bud-to-cap stage transition during early tooth development is a time when the tooth-inducing potential becomes restricted to the mesenchyme. Several key genes, expressed in the mesenchyme at this stage, are an absolute requirement for the progression of tooth development. These include the transcription factors Msx1 and Pax9. The inductive potential of tooth mesenchyme cells is a key requisite for whole-tooth bioengineering and thus identification of cells that can retain this property following expansion in culture is an important as yet unresolved, goal. We show here that in-vitro culture of embryonic human tooth mesenchyme cells and SHED cells express low levels of PAX9 and MSX1 and …

Le Granulome éosinophile des maxillaires: une zone radiotransparente qui n'est pas toujours pathognomique d'une véritable pathologie

Le Granulome éosinophile des maxillaires: une zone radiotransparente qui n’est pas toujours pathognomonique d’une véritable pathologie.

Rat model of burn wound healing: Effect of botox

Animal models of burn play a crucial role in studying the mechanisms of burn wound progression and the factors that regulate various stages of healing. In this study, using a rat model, we assessed the effect of Botox in the healing process through parameters like transepidermal water loss (TEWL), histological alterations, transforming growth factor beta (TGF-beta1) and tumor necrosis factor alpha (TNF-alpha). Fifty Sprague-Dawley rats were inflicted with 5 cm2 second degree burn and divided into 2 groups; one group was injected intralesionally with Botox and the other with saline. Daily observation and transepidermal water loss measurement were performed. Biopsies were taken on days 0, 3, …

Modification of the peptides ANP and oxytocin in the rat hypothalamic paraventricular nucleus during the physical exercises.

Mandibulary Eosinophilic Granuloma: Case Series

Eosinophilic granuloma is a proliferation of Langerhans cells in the bones that most commonly involving the skeletal system. The disease generally affects children or young adults with predominance in males and it is characterized by a single or multiple skeletal lesions. This study describes a few cases of mandibular Eosinophilic Granuloma and the effects of surgical curettage of lesions

Le remaniement de la Matrice Métallo-Proteinase est impliquée dans l'Odontogènese? Etude du MMP-9

Horizontal and Vertical Reconstruction of the Severely Resorbed Maxillary Jaw Using Subantral Augmentation and a Novel Tenting Technique with Bone from the Lateral Buccal Wall

Aim The aim of this study was to evaluate the effect of using the lateral wall bone in sinus lifting two-dimensional reconstruction on bone augmentation.Patients and Methods Ten patients affected by class V or VI maxillary atrophy with less than 3 mm of residual horizontal ridge were selected. Using a piezo-ultrasonic surgery tip bony lateral wall was cut. To expose native bone to the bone graft, multiple perforations, made through the cortical plate of the recipient site with a round bur. Once the bony buccal wall was adjusted it was fixed away from the ridge with two 1.5 x 13 mm bone fixation screws. Deficiencies created between the bony buccal wall and the ridge was filled with a mineral…

Regulation of odontogenic gene expression in dental stem cells

Italian Journal of Anatomy and Embryology, Vol 115, No 1/2 (Supplement) 2010

EXPERIMENTAL COLITIS: CHEMICAL AND BACTERIAL INDUCTION

Expression and regulation of PAX-9 and MSX-1 in embryonic and post-natal dental stem cells.

Introduction: Msx1and Pax9, are genes expressed in mesenchymal cells during the odontogenesis that are required for tooth formation. Our investigation aimed to study the expression of these genes in two sources of dental mesenchymal cells. Stem Cells from Human Exfoliated Deciduous teeth (SHED) and Human Embryonic Dental Mesenchymal Cells (HTM) were used. Regulation of gene expression in these cells by FGF, Wnt, BMP4 and Shh signalling pathways together with the effects of tooth matrix proteins (EMD) was investigated. Materials and Methods: Cells were grown in 6 well plates in 3 ml MSCGM medium (Lonza) containing Glutamax, 10% FBS and gentamicin Sulfate. Cells were grown to confluence and w…

Heat Shock Proteins Alterations in Rheumatoid Arthritis

Rheumatoid arthritis (RA) is a chronic inflammatory and autoimmune disease characterized by the attack of the immune system on the body’s healthy joint lining and degeneration of articular structures. This disease involves an increased release of inflammatory mediators in the affected joint that sensitize sensory neurons and create a positive feedback loop to further enhance their release. Among these mediators, the cytokines and neuropeptides are responsible for the crippling pain and the persistent neurogenic inflammation associated with RA. More importantly, specific proteins released either centrally or peripherally have been shown to play opposing roles in the pathogenesis of this dise…

Role of matrix metalloproteases 2, 9 (MMP-2, -9), orphanin FQ and others peptides in vascular tone of human umbilical cord at term

Umbilical cord is the structure that connects the fetus to the placenta. Umbilical cord at term is made of three vessels surrounded by the Wharton’s jelly, a mucous connective tissue rich in glycosaminoglycans, proteoglycans and different types of collagens and other microfibrils, which are responsible for its elasticity and its mechanical properties.Umbilical cord seems to lack any capillaries or lymphatics and umbilical vessels are unique in lacking any innervation; thus endothelial cells may play the major role in the local control of the blood flow. Objectives. In this paper we investigated in human umbilical cord at term the expression of some peptides involved in the regulation of the…

Expression of beta-Endorphin and Caspase-9 in orthodontically treated dental pulp

HIV-Infected Children and Nutrition: The Friend and The Foe

The impact of nutrition on HIV-infected children has been evaluated in multiple studies. Our review of the current trends of nutrition-related studies revealed that the focus has moved from simply the disease consequences of HIV to ensuring that antiretroviral therapy-treated children are well nourished to ensure growth and development. This update aims to present the state of the art regarding nutrition of HIV-infected children and the real potential for nutrition to serve as a dynamic therapy in this group. Recent World Health Organization reports indicate that the HIV/AIDS disease is curbing in incidence worldwide despite the high 1.8 million children, less than 15 years, reported in 201…

The Bio-Molecular Dynamics of Dental Pulp in Different Clinical Scenarios

Dental pulp (DP) is a very dynamic tissue both in health and in disease. When exposed to stressors and pathological conditions. It undergoes a complex series of biological reactions whereby alterations affect the pulp tissue at tissue cellular and molecular levels. The aim of this review is to update the reader on the various bio-molecular alterations in the dental pulp under different clinical conditions: orthodontic treatment (OT), caries, pulpitis and others. The morphological changes in the composition of the DP rang from the reversible remodeling to apoptosis and sometimes necrosis. Many apoptotic factors are involved like Bcl2, Bax and the significant increase in Caspases 9 and 3, as …

MEK1 Required for Invasive Growth of mIMCD3 renal Cells in a 3D Collagen Matrix

Branching morphogenesis is an essential process during kidney development: This process controls epithelialization of the metanephric mesenchyme during the induction of branching structures and in this way determines the number of nephrons. Various grow factors, such HGF, have been implicated in branching morphogenesis in renal cells, including mIMCD3 ( murine inner medullary collecting duct) and MDCK cells. Growth factors which induce branching morphogenesis in renal cells also activate the MEK1/ERK pathway. The authors analyzed the potential role that this pathway might play in branching morphogenesis. The authors show that PD98059, a specific inhibitor of MEK1,inhibits branching of mIMCD…

The role of new neurocrine and neuroendocrine messengers on the regulation of the function of major salivary glands in some mammals. An immunohistochemical study

HSP60 activity on human bronchial epithelial cells

HSP60 has been implicated in chronic inflammatory disease pathogenesis, including chronic obstructive pulmonary disease (COPD), but the mechanisms by which this chaperonin would act are poorly understood. A number of studies suggest a role for extracellular HSP60, since it can be secreted from cells and bind Toll-like receptors; however, the effects of this stimulation have never been extensively studied. We investigated the effects (pro- or anti-inflammatory) of HSP60 in human bronchial epithelial cells (16-HBE) alone and in comparison with oxidative, inflammatory, or bacterial challenges. 16-HBE cells were cultured for 1–4 h in the absence or presence of HSP60, H2O2, lipopolysaccharide (…

S2-P114 MAXILLARY BONE NECROSIS FOLLOWING THE USE OF FORMALEHYD-CONTAINING PASTE. THREE CASE REPORTS

Caspase-9 and beta-endorphin expression in human dental pupl subjected to orthodontic traction

Gemma Dentale: studio immunoistochimico

Données immunohistochimiques sur la presence de la Periostine dans le bourgeon dentaire dans un foetus humain de 13 semaines

Salivary gland proteins alterations in the diabetic milieu

AbstractSalivary glands are considered the chief exocrine glands of the mouth and physiologically contribute to the maintenance of the homeostasis of the oral cavity. They consist of the parotid, submandibular and sublingual glands, which come in pairs and are collectively called the major glands, and the minor glands, which are much smaller and are dispersed throughout the buccal cavity. Salivary glands are distinguished by their size, amount of saliva secretion and their location in the oral cavity. Salivary glands pathophysiology has been a subject of interest in various worldwide metabolic disorders, including diabetes mellitus. Diabetes mellitus (DM), a global health concern, with a pa…

The chaperone system in cancer therapies: Hsp90

AbstractThe chaperone system (CS) of an organism is composed of molecular chaperones, chaperone co-factors, co-chaperones, and chaperone receptors and interactors. It is present throughout the body but with distinctive features for each cell and tissue type. Previous studies pertaining to the CS of the salivary glands have determined the quantitative and distribution patterns for several members, the chaperones, in normal and diseased glands, focusing on tumors. Chaperones are cytoprotective, but can also be etiopathogenic agents causing diseases, the chaperonopathies. Some chaperones such as Hsp90 potentiate tumor growth, proliferation, and metastasization. Quantitative data available on t…

Probiotics Can Cure Oral Aphthous-Like Ulcers in Inflammatory Bowel Disease Patients: A Review of the Literature and a Working Hypothesis

Dysbiosis has been associated with the onset of several chronic autoimmune or inflammatory pathologies (e.g., inflammatory bowel diseases—IBD), because of its primary role in the establishment of a chronic inflammatory process leading to tissue damage. Inflammatory bowel diseases can even involve areas far away from the gut, such as the extraintestinal manifestations involving the oral cavity with the onset of aphthous-like ulcers (ALU). Studies carried out on animal models have shown that intestinal dysbiosis may be related to the development of autoimmune diseases, even if the mechanisms involved are not yet well known. The aim of this paper is to verify the hypothesis that in inflammator…

Nerve growth factor and burn wound healing: Update of molecular interactions with skin cells

Burn wound healing is a very intricate and complex process that conventionally includes three interrelated and overlapping stages of hemostasis/inflammation, proliferation and remodeling. This review aims to explore the molecular interactions of NGF with the most prominent cell types in the skin and their respective secretory products during wound healing, particularly burn wound healing. Different types of cells such as, nerve cells, endothelial cells, mast cells, macrophages, neutrophils, keratinocytes and fibroblasts all come into play through a plethora of cytokines and growth factors including nerve growth factor (NGF). NGF is a pleiotropic molecule that exerts its effects on all the a…

Preliminary observations on the immunohistochemical distribution of nNOS in the development of human foetus eye

Immunohistochemical distribution of NOS Isoform in the Embryofoetal structures of Human encephalon

CYTOLOGY AND CLINICAL SPECTRUM OF SEXUALLY TRASMITTED INFECTIONS IN LEBANESE WOMEN ARE REVEALED BY PAP SMEAR: A CROSS-SELECTION STUDY FROM 2002-2006

OBJECTIVE: To study the cytology profile of cervical smears and the respective prevalence and incidence of certain cervico-vaginal infections detectable by routine Pap smear cytology in Lebanese women from 2002 to 2006. METHODS: Pap smear cytology results were compiled from the archives of the “Institut National de Pathologie” for the period extending from 2002 until 2006. This study covered 118,230 cervical specimens obtained from Lebanese women attending clinics and hospitals in all the five districts of Lebanon; prevalence and incidence rates for infections detectable by routine Pap smear examination were determined. RESULTS: A rise in prevalence of these infections by 2.1% (2,555) from …

Histochemical and ultrastructural remarks of the urinary tract in some mammals.

CAPSAICIN STIMULATION AND NEUROGENIC EXPERIMENTALLY INDUCEND INFLAMMATION OF THE HUMAN LABIAL MUCOSA

TWENTY YEARS' HISTORY OF IMMUNOHISTOCHEMICAL MAMMALS DENTAL PULP STUDIES

Cell Differenciation markers in human umbilical cord; an immunohistochemical study

The full-term human umbilical cord is made of three blood vessels ( two arteries and a vein) surrounded by the Wharthon's jelly, a soft cennective tissue made of new mesenchymal stromal cells and an abundant extracellular matrix. Both endothelial and Wharton's jelly are versdatile in their differenciation potential;tewhy have shown to be able to differentiate into sevaral cell lineages. Since the differentiation potential of these cells is very wide, we thought it could be interesting to investigate the expression of several cell differentiation markers in the different regions of human umbilical cord. Immunohistochemistry showed the expression of E- Cadherin and cytokeratyns in the amnioti…

SUPERIOR MESENTERIC ARTERY SYNDROME: CLINICAL, ENDOSCOPICAL AND RADIOLOGICAL CORRELATIONS ACCORDING TO A SINGLE-CENTER EXPERIENCE

Background and aim: The superior mesenteric artery (SMA) syndrome is a rare entity presenting with upper gastrointestinal tract obstruction and weight loss, due to the compression of the third part of duodenum between the aorta and the SMA. Studies to determine the optimal methods of diagnosis and treatment are essential. This study aims to analyze the clinical presentation, the doagnosis and the management of this syndrome. Material and medthods: Over a 2-year (2013-2014), 8 cases of SMA syndrome (out of 2074 esophago-gastro-duodenoscopies, EGDS) were initially suspected through EGDS. Therefore, these patients performed computed tomography (CT) scan to confirm the diagnosis. Once the diagn…

CB1 receptor expression in pancreatic islet of the obese and diabetes Zucher rats

Molecular Mechanisms Leading from Periodontal Disease to Cancer

Periodontitis is prevalent in half of the adult population and raises critical health concerns as it has been recently associated with an increased risk of cancer. While information about the topic remains somewhat scarce, a deeper understanding of the underlying mechanistic pathways promoting neoplasia in periodontitis patients is of fundamental importance. This manuscript presents the literature as well as a panel of tables and figures on the molecular mechanisms of Porphyromonas gingivalis and Fusobacterium nucleatum, two main oral pathogens in periodontitis pathology, involved in instigating tumorigenesis. We also present evidence for potential links between the RANKL–RANK signaling axi…

Human Umbilical Cord at Term. Immunohistochemical expression of MMP-2, MMP-9, Orphanin F/Q and others Peptides involved in the regulation of vascular tone

B-Endorphin in Human Dental Pulp During Orthodontic Treatments

Modulation of mucin 2 and mucin 3 in colitis induced by iodoacetamide and enteropathogenic bacteria in rats

The iinate and acquired immune systems are both implicarted in the etiology of Inflammatory Bowel Disease (IBD) in addition to the genetic predisposition, the environmental factors and the intestinal flora covering the mucosa. A defect in the mucous covering will lead to an invasion of pathogens and stimulation of the immuune response with aberrations of mucin 2, the major mucin of the mucous layer. Aim: this study aims to assess the modulation of colonic MUC 2 and MUC 3 in a arat model of IBD induced by a combination of iodoacetamide and enteropatogenic E. Coli. Methods: 78 sprague-Dawley female rats were divided into 4 groups. Each group was subjeceted, on a basis, to a rectal injection o…

Immunohistochemical and transcriptional expression of the matrix metalloproteinases MMP-2 and MMP-9 in normal and pathological human oral mucosa

The oral cavity is exposed to chronic or recurrent, physical and chemical trauma that could lead to mucosal reactions (e.g. hyperplasia, dysplasia and tumors). The objective of this study is to investigate the expression and the possible changes of the two matrix metalloproteinases MMP-2 and MMP-9 in normal and pathological human oral mucosa samples. Normal oral mucosa samples and three different types of pathological conditions (hyperplasia, dysplasia and carcinoma) were used for this study. Immunohistochemical analysis was used to evaluate protein expression for the two enzymes, while Reverse Transcription ? Polymerase Chain Reaction (RT-PCR) was used to investigate gene expression. Image…

Le granulome éosinophile des maxillaires: une zone rediotrasparente qyi n'est pas toujours pathognonomique d'une véritable pathologie

PEMPHIGUS VULGARIS IN A MEDITERRANEAN YOUNG ADULT MANIFESTING AS DESQUAMATIVE GINGIVITIS

The Chaperone System in Salivary Glands: Hsp90 Prospects for Differential Diagnosis and Treatment of Malignant Tumors

Salivary gland tumors represent a serious medical problem and new tools for differential diagnosis and patient monitoring are needed. Here, we present data and discuss the potential of molecular chaperones as biomarkers and therapeutic targets, focusing on Hsp10 and Hsp90. The salivary glands are key physiological elements but, unfortunately, the information and the means available for the management of their pathologies, including cancer, are scarce. Progress in the study of carcinogenesis has occurred on various fronts lately, one of which has been the identification of the chaperone system (CS) as a physiological system with presence in all cells and tissues (including the salivary gland…

ASSOCIATION BETWEEN HELYCOBACTER PYOLRI INFECTION AND PATHOLOGICAL ORAL MANIFESTATIONS

DATA FROM LITERATURE ARE CONTROVERSAL REGARDING THE PRESENCE OF bELICOBACTER PYLORI (HP) IN DENTAL PLAQUE AND ITS ASSOCIATION WITH GASTRIC INFECTION. oNE OF THE POSSIBLE MECHANISMS SUGGETSED FOR RE-INFECTION IS THE RECOLONIZATION WITH HP DENTAL PLAQUE. THE PURPOSE OF THIS REVIEW WAS TO DETERMINE WHETHER DENTAL PLAQUE, POOR ORAL HYGIENE, AND PERIODONTAL DISEASE WERE RISK FACTORS FOR HP INFECTION.

Medici e Medichesse ebrei nella Sicilia del 1400

P23-Le remaniement de la Matrice metallo-Proteinase est impliquée dans l'Odontogènese? E'tude du MMP-9

Natriuretic peptide system expression in murine and human submandibular salivary glands: a study of the spatial localisation of ANB, BNP, CNP and their receptors

AbstractThe natriuretic peptide (NP) system comprises of three ligands, the Atrial Natriuretic Peptide (ANP), Brain Natriuretic peptide (BNP) and C-type Natriuretic peptide (CNP), and three natriuretic peptide receptors, NPRA, NPRB and NPRC. Here we present a comprehensive study of the natriuretic peptide system in healthy murine and human submandibular salivary glands (SMGs). We show CNP is the dominant NP in mouse and human SMG and is expressed together with NP receptors in ducts, autonomic nerves and the microvasculature of the gland, suggesting CNP autocrine signalling may take place in some of these glandular structures. These data suggest the NP system may control salivary gland funct…

Immunohistochemical localization of MMP-9 and Orphanin in some sample of dental pulp.

CORDON OMBILICAL.NOUVELLES SUR L'ACETYLCHOLINE

Dental pulp in mature human teeth: morphological alterations and metalloproteinases-2 and -9, Annexin-5, BCL-2, and iNOS modulation.

Tooth replantation, as a treatment concept, has been subject to controversies regarding the mechanism as well as the various parameters underlying this process. This work aimed to study time-related changes in the pulp of replanted mature human premolars through the changes in the levels of certain factors involved in the underlying mechanisms of pulpal tissue healing after replantation. Eleven experimental mature teeth were extracted, immediately replanted in the original socket and left without any other intervention for 1, 2, 3 and 12 weeks before re-extraction. Three premolars served as control. All specimens were subject to histological analysis and the levels of MMP-2, MMP-9, Annexin …

A novel therapeutic approach to colorectal cancer in diabetes: role of metformin and rapamycin

International audience; The link between colorectal cancer (CRC), diabetes mellitus (DM) and inflammation is well established, and polytherapy, including rapamycin, has been adopted. This study is a novel approach that aimed at assessing the effect of a combination therapy of metformin and rapamycin on the control or prevention of CRC in diabetic animals, in presence or absence of probiotics. Fifty NOD/SCIDs male mice developed xenograft by inoculating HCT116 cells. They were equally divided into diabetics (induced by Streptozotocin) and non-diabetics. Metformin was given in drinking water, whereas rapamycin was administered via intra-peritoneal injections. Probiotics were added to the doub…

Hsp60 as a Novel Target in IBD Management: A Prospect

Inflammatory bowel disease (IBD) encompasses various pathological conditions similar but distinct that share a multifactorial etiology, including involvement of the intestinal barrier function, the immune system, and intestinal microorganisms. Hsp60 is a chaperonin component of the chaperoning system, present in all cells and tissues, including the intestine. It plays important roles in cell physiology outside and inside mitochondria, its canonical place of residence. However, Hsp60 can also be pathogenic in many conditions, the Hsp60 chaperonopathies, possibly including IBD. The various clinico-pathological types of IBD have a complicated mix of causative factors, among which Hsp60 can be …

GIOVANNI GORGONE" THE NESTOR OF ANATOMISTS"

Orthodontic Stress BCL-2 Nodulation and Human Odontoblast's Survival

Colorectal Carcinogenesis: Role of Oxidative Stress and Antioxidants

One of the contributory causes of colon cancer is the negative effect of reactive oxygen species on DNA repair mechanisms. Currently, there is a growing support for the concept that oxidative stress may be an important etiological factor for carcinogenesis. The purpose of this review is to elucidate the role of oxidative stress in promoting colorectal carcinogenesis and to highlight the potential protective role of antioxidants. Several studies have documented the importance of antioxidants in countering oxidative stress and preventing colorectal carcinogenesis. However, there are conflicting data in the literature concerning its proper use in humans, since these studies did not yield defin…

DISMICROBISM IN INFLAMMATORY BOWEL DISESE AND COLORECTAL CANCER: CHANGES IN RESPONSE OF COLOCYTES

Patients with inflammatory bowel disease (IBD) have an increased risk of 10%-15% developing colorectal cancer (CRC) that is a common disease of high economic costs in developed countries. the CRC has been increasing in recent years and its mortality rates are very high. Multiple biological and biochemical factors are responsible for the onset nad progression of this pathology. moreover, it appears absolutely necessary to investigate the environmental factors favoring the onset of CRC and the production of colonic healt. the gut microflora, or microbiota, has an extensive diversity both quantitatively and qualitatively. in utero, the intestine of the mammalian fetus is sterile. Al birth, the…

Prevalence of IL-1B+3954 and IL-1a-889 Polymorphisms in The lebanese Population and its association with the severity of adult chronic periodontitis

Pro-inflammatory cytokine, i.e., IL-1 mediate the inflammatory response and are genetically regulated in periodontal diseases. Strong association was found between the composite genotype allele 2 of IL-1β+3954 and IL-1α-889 and severe chronic periodontitis. The aim of this study is to determine the prevalence of IL-1β+3954 and IL-1α-889 polymorphism in a group of Lebanese individuals of homogeneous ethnicity and the possible association between genotype positive individuals and the severity of periodontal disease. One hundred and fifty-seven patients aged 53.29±13.13 years participated in the study. Subjects were classified as follows: 1) healthy subjects with no attachment loss >1mm and no…

Immunohistochemical expression and distribution of orexin, orphanin and leptin in the major salivary glands of some mammals

Abstract: The aim of the study was to assess the involvement of apoptotic factors, cytokeratins and metalloproteinase- 9 in the histogenesis of both Epithelialized Gingival Lesions (EGL) and Periapical Lesions (PAL). 55 consecutive patients, 30 with PAL and 25 with EGL, were selected for the study after clinical and radiological examinations. The PAL patients had severe periapical lesions and tooth decay with exposure of the pulp chamber. All PAL and EGL biopsies were surgically extracted, fixed in 10% buffered formalin, and processed for routine light microscopy. Ten biopsies of each category were processed for immunohistochemistry (IHC). Serial paraffin sections were stained by IHC with a…

CANALAR REPROCESSING WITH NI-TI INSTRUMENTS: OUR EXPERIENCE ITALIAN JOURNAL OF ANATOMY AND EMBRIOLOGY

Oral capillaroscopy: our experience

Capillaroscopy has now definitely become an important and indispensable exam in the area of diagnosing vasculopathies and in particular micro angiopathies. The type of damages connected to these anatomy pathologies regarding the actual micro vascular unit work in a functional pathogenic of an extreme importance, in conditioning the course and the eventual aggravation of micro angiopathies properly called vasculity and also of macro angiopaties. In all, this underlines the great importance achieved by using certain instrumental exams which complements the clinical research, exactly because of the valid elements which have been given for a complete and precise morphological valuation for the …

Nouvelles utilisations du collagène de type I dans la determination du potentiel métastatique des cellules tumorales: etudes morphologique et biochimique

Re-Emerging Vaccine-Preventable Diseases in War-Affected Peoples of the Eastern Mediterranean Region—An Update

For the past few decades, the Eastern Mediterranean Region has been one area of the world profoundly shaped by war and political instability. On-going conflict and destruction have left the region struggling with innumerable health concerns that have claimed the lives of many. Wars, and the chaos they leave behind, often provide the optimal conditions for the growth and re-emergence of communicable diseases. In this article, we will highlight three of the major re-emerging vaccine preventable diseases cholera, measles, and polio, in four countries of the Eastern Mediterranean Region that are currently affected by war leading to a migration crisis: Iraq, South Sudan, Syria, and Yemen. The re…

Localisation immunohistochemique du factuer natriuretique atriale du tissu muqueux mur du Cordon Ombelical humain après naissance et dans la Pulpe Dentaire.

Twenty years' history of immunohistochemical mammals dental pulps studies.

Functional Aspects of Adrenocholinergic and Peptidergic Innervation about Prostate and Seminal Vesicles in Mammals: A Review

This study was aimed to explain the functional role of mediation in the organs of the male reproductive system (prostate and seminal vesicles of rat and hamster), representing throught the visualization of adrenergic fibres (Falck Method), cholinergic fobres (Martinez-Rodriguez method) and by immunohistochemical methods to detect the P substance and the CGRP (PAP) in glandular structures. The adrenocholinergic and peptidergic mediators have a growing interest for their synergic action in the control of contractility, vasodilatation and most likely, of the secretory activity of the epithelium, probably expressed by andocrine-paracrine mechanisms.

Expression of Remodelling Enzymes (MMP-2, MMP-9) in Normal and Pathological Oral Mucosa Samples

Introduction. The oral cavity contains many structures compressed in a small area. It is exposed to chronic or recurrent, physical and chemical trauma leading to mucosal reactions (e.g. hyperplasia, dysplasia and tumors). For a long time, our group has been studying the expression of some metalloproteinases (MMP-2, MMP-9) in healthy and pathological human oral mucosa through immunohistochemistry and RT-PCR to investigate the entity of the remodelling process in the different cases. Matherials and Methods. In this paper we investigate a sample of normal oral mucosa and four pathological samples (mucocele, fibroma, dysplasia and carcinoma). We identified the localization of MMP-2 and MMP-9 th…

COX- Expression in the inflamed gingival tissue:Immunohistochemistry,Cytochemistry and Biochemistry test.

Prostaglandins (PG) are chemical mediators for most physiological and pathological responses ,including cytoprotection of the gastric mucosa, blood coagulation,inflammation and pain (1,2,3). E2 Prostaglandins (PG2) play an important role of pathogenesis of periodontal diseases, since PG2 determines vasodilatation and increased vascular permeability, oedema and erythema.Therefore,it is not surprising to find high levels of PG2 in inflamed gingival tissues.Indeed ,in inflamed gingival tissue,PG2 levels of the Gingival Fluid (GCF) are known to be higher in periodontitis than in healthy gingiva.Moreover ,in different parodontitis the high PG2 level of the GCF is related to periodontal ligament …

Chaperonin Hsp60 and Cancer Therapies

The heat shock protein 60 (Hsp60) is a chaperonin belonging to the chaperoning (chaperone) system that typically contributes to protein homeostasis inside mitochondria, but also plays various non-canonical roles unrelated to protein quality control beyond the organelle. Chaperonopathies are disorders in which chaperones play an etiologic-pathogenic role and contribute to the onset/progression of disease. Hsp60 chaperonopathies by mistake are diseases in which the chaperonin is apparently normal (as far as it can be determined with current methodologies) but it actively contributes to pathology, for example in certain types of cancer, and autoimmune and chronic inflammatory disorders. In cer…

Cell differentiation markers in human umbilical cord; an immunohistochemical study

The full-term human umbilical cord is made of three blood vessels (two arteries and a vein) surrounded by the Wharton’s jelly, a soft connective tissue made of few mesenchymal stromal cells and an abundant extracellular matrix. Both endothelial and Wharton’s jelly cells are versatile in their differentiation potential; they have shown to be able to differentiate into several cell lineages. Since the differentiation potential of these cells is very wide, we thought it could be interesting to investigate the expression of several cell differentiation markers in the different regions of human umbilical cord. Immunohistochemistry showed the expression of E-cadherin and cytokeratins in the amnio…

Vent'anni di storia sullo studio immunoistochimico delle polpe dentali di mammiferi Twenty years'history of immunohistochemical mammals dental pulp studies

Cytocheratins Identification in Human Umbilical Cord at Term.

Introduction. Cytokeratins (Cks) are the proteins that constitute intermediate filaments. The pattern of expression of cytokeratins is frequently organ or tissue specific and depends mainly on the type of epithelium, the level of differentiation and the stage of development. With this study we tried to make light on the pattern of expression of a wide range of CKs in human umbilical cord at term. Materials and methods. We investigated the expression of CKs through immunohistochemistry and RT-PCR assay. Results. AE1/AE3 antibody reacts with the basic CKs 1, 2, 3, 4, 5, 6, 7 and 8, and with the acidic CKs 10, 13, 14, 15, 16 and 19. The reactivity for this antibody is localized in the amniotic…

THE CHAPERONE SYSTEM IN SALIVARY GLAND DEVELOPMENT

The chaperone system (CS) canonical function is to maintain protein homeostasis. Proper folding of nascent peptides is crucial in developing tissue. The chief components of the CS are 95th National Congress of the Italian Society for Experimental Biology | Trieste, Italy, 12-15 April 2023Non-commercial use only the molecular chaperones, which play key roles in development as indicated by their presence in embryonic tissue as early as at two-cell stage. However, scarce information on the CS in developing tissues is available, especially at advanced stages of embryogenesis and its role is not fully understood. In our previous study, we reported the presence of molecular chaperones in the duct…

EFFECTS OF A FERRIC OXALATE DENTIN DESENSITIZER: SEM ANALYSIS