Intracellular distribution of the La antigen in CV-1 cells after herpes simplex virus type 1 infection compared with the localization of U small nucl…

1989

The La antigen is known to associate, at least transiently, with a series of small nuclear and cytoplasmic ribonucleoprotein particles (snRNPs and scRNPs), e.g. U1 and U6 snRNPs. In CV-1 cells a monoclonal antibody (MAb), directed against the La protein (La1B5), immunostained intranuclear speckles. These speckles were found to co-localize with speckles that were stained by MAbs directed against either all U snRNPs or only against U1 snRNPs. Two h after infection of CV-1 cells with herpes simplex virus type 1 (HSV-1) (strain HFEM) the staining of nuclear speckles with the anti-La MAb disappeared and the La protein was found quantitatively in the cytoplasm. In contrast nuclear speckles remain…

Numerical analysis of lamellar thickness distributions

1989

Etude comparative des distributions d'epaisseur de cristallites dans des polyethylenes de masse molaire 10000-100000 cristallises presentant des distributions etroite, bimodale, asymetrique ou large, par microscopie electronique, diffusion RX centrale et spectroscopie Raman. Variations des resultats avec la morphologie des cristallites et surtout avec la nature de la distribution

Nachweis von Markembolien wÄhrend Oberschenkelmarknagelungen mittels transösophagealer Echokardiographie

1989

Kardiopulmonale Komplikationen nach Marknagelosteosynthesen, insbesondere am Oberschenkel, sind bekannt. WAhrend der Nagelung einer Oberschenkelquerfraktur mit einem, geschlossenen distalen Fragment konnten durch transsophageale Echokardiographie im rechten Herzen durchstromende echogene Partikel nachgewiesen werden. Das intrakardiale Auftreten dieser Partikel stand in engem zeitlichen Zusammenhang mit dem Aufbohren der femoralen Markhohle und kann als Nachweis verschleppter Marksubstanz gedeutet werden. Die intraoperative Echokardiographie bietet sich an, Untersuchungen uber die Pathophysiologie kardiopulmonaler VerAnderungen nach Marknagel osteosynthesen durchzufuhren und die EffektivitAt…

Determination of fluoride in rocks, soils, and fluoride-bearing minerals by separation using direct distillation in sulfuric acid medium and spectrop…

1989

Abstract An analytical method for fluoride determination in geological samples is proposed. It is based on a previous fluoride separation by distillation with a Teflon reactor. The variables that influence distillation quantitation (temperature, time, and acid concentration) are optimized. The influence of some species on fluoride recovery during distillation are studied, and conditions to avoid this influence are suggested. Fluoride determination is carried out by means of a spectrophotometric method [La(III)-F−-Alizarin complexone system] (N. T. Crosby, A. L. Dennis, and J. G. Stevens, Analyst (London), 1968, 93, 643–652). The method is applied to the analysis of soils, rocks, and fluorid…

Spaces of typen on partially ordered sets

1989

This paper contains a generalized approach to incidence geometry on partially ordered sets. A difference to the usual geometrical concepts is that points may have different size. Our main result states that a large class of spaces allows lattice theoretic characterizations. Especially, a generalized version of the Veblen-Young axiom of projective geometry has a lattice theoretic equivalent, called then-generation property (which is a generalization of the ‘Verbindungssatz’). Modularity and distributivity of a lattice of subspaces are reflected in the underlying space. Finally we give specializations and examples.

The determination of haemoglobin as cyanhaemiglobin or as alkaline haematin D-575. Comparison of method-related errors.

1989

In order to compare the accuracy of haemoglobin (Hb) determination methods, the commonly used cyanhaemiglobin (HiCN) method and the recently developed alkaline haematin D-575 (AHD) method (R. Zander, W. Lang & H. U. Wolf (1984) Clin. Chim. Acta 136, 83-93; H. U. Wolf, W. Lang & R. Zander (1984) Clin. Chim. Acta 136, 95-104) were tested with respect to method-related errors such as plasma, cell, and Hb errors. Both methods yield a series of more or less significant errors which generally lead to an overestimation of the Hb concentration in the order of 1%. However, in all three cases of plasma errors, i.e. normal plasma error, plasma error in lipaemic blood, and plasma error in bilirubinaemi…

Major histocompatibility complex class I-restricted activation of cloned T cells by a soluble protein in the absence of accessory cells.

1989

A T-cell clone, 10BK.1, was established from the draining lymph nodes of (B10 x B10.BR)F1 mice immunized with ovalbumin (OVA) according to standard protocols. Upon coculture with the antigen, 10BK.1 cells reacted by production of lymphokines and by proliferation despite the absence of additional antigen-presenting cells. These T cells do not express major histocompatibility complex (MHC) class II molecules on the cell surface as assessed on the basis of several criteria: by cytofluorometric analysis I-A and I-E determinants were not detectable; 10BK.1 cells could not act as antigen-presenting cells for long-term-cultured MHC class II-restricted T-cell clones; and monoclonal antibodies direc…

Thin Liquid Films. Edited byI. B. Ivanov. Marcel Dekker Inc, New York 1988. 1160 pp., bound, US $ 295.—ISBN 0-8247-7763-8

1989

A self-centralizing characteristic subgroup

1989

AbstractIn this note we introduce a self-centralizing characteristic subgroup, associated with quasinilpotent injectors, of a finite group.

Scaled chrysophytes (Chrysophyceae and Synurophyceae) from national park lakes in southern and central Finland

1989

A total of 34 scaled chrysophyte taxa (18 Mallomonas spp., 6 Synura spp., 2 Chrysosphaerella spp., 6 Spiniferomonas spp. and 2 Paraphysomonas spp.) were recorded by SEM techniques in 61 samples from 59 lakes locating in 8 national parks of southern and central Finland in July 1987. Most of the lakes were small forest and peat bog lakes with acid (pH 4.6–7.2) and soft-water and with variable water colour (10–350 mg Pt/l). The number of taxa per lake varied from 0 to 15 and it correlated very significantly with the water pH. The species structure was rather typical for the oligotrophic and acid lakes. Besides the eurytypic and common species like Mallomonus caudata (fr. 72.1% of lakes), M. cr…

Influence of bacteria on the metabolite pattern of phenol in zebra fish (Brachydanio rerio)

1989

Summary An investigation was performed on the potential influence of bacteria in the medium on the metabolism of phenol in zebra fish. The results obtained in vitro and in vivo show that bacteria may influence biotransformation studies of xenobiotics by fish in different manner. First, it was shown that phenol conjugates were deconjugated. Hydrolysis of phenyl glucuronide was observed both in vitro and in vivo. Deconjugation of phenyl sulphate was found to a minor extent merely under in vitro conditions. Second, the xenobiotic itself, in this case phenol, was degradated to CO2. The proportion did not lead to a significant removal of the test compound of the fish. Zusammenfassung Einflus von…

Astrophysical bound on the majoron-Higgs-boson coupling

1989

Abstract We show that the coupling of the “standard” Higgs boson to majorons, that could lead to a very fast decay of the neutral Higgs scalar to invisible modes, can be bounded using astrophysical arguments. We discuss the relevance of this bound for low-energy phenomenology related to majoron production. The bound so obtained may also jeopardize the stability of the VEV hierarchy in the doublet and triplet majoron models if the mass of the top quark is less than the W mass. A similar analysis may be applied to other models which exhibit Goldstone-or pseudo-Goldstone-bosons in the spectrum.

A comparison of the Raman LAM and electron microscopy in determining crystallite thickness distributions: Polyethylenes with narrow size distributions

1989

Bon accord entre les resultats obtenus pour des polyethylenes cristallises a 118 o C pendant 2 et 20 minutes lorsqu'on prend en compte l'inclinaison de la chaine et pour des polymeres a distribution etroite

Energy requirement and kinetics of transport of poly(A)-free histone mRNA compared to poly(A)-rich mRNA from isolated L-cell nuclei.

1989

ATP-promoted efflux of poly(A)-rich RNA from isolated nuclei of prelabeled mouse lymphoma L5178y cells has an activation energy of 51.5 kJ/mol, similar to that found for the nuclear envelope nucleoside triphosphatase (48.1 kJ/mol) assumed to be involved in mediating nucleocytoplasmic transport of at least some RNA. Here we show that efflux of two specific poly(A)-rich mRNAs (actin and beta-tubulin) from isolated L-cell nuclei is almost totally dependent on the presence of ATP, while efflux of poly(A)-free histone mRNA (H4, H2B, and H1) also occurs to a marked extent in the absence of this nucleotide. Measurements of temperature dependence of transport rate revealed an activation energy of 5…

Fast and nonselective on-line mass separation of neutron-rich isotopes produced in proton-induced fission

1989

Abstract On-line mass separation of short-lived radionuclides, based on the use of thermalized primary ions from nuclear reactions, termed as an ion guide method, has recently been widely applied in the spectroscopy of both proton-rich and neutron-rich exotic nuclei. The technique has been found to be applicable to all elements and it has allowed the detection of the mass-analyzed activities with half-lives as short as 0.1 ms. The ion guide isotope separator on-line, IGISOL, has recently been used to discover over ten new short-lived isotopes or isomers produced in the nearly symmetric fission of uranium induced by 20 MeV protons. In addition to applications in nuclear-structure physics and…

Polypeptides controlling hematopoietic blood cell development and activation

1989

Colony-stimulating factors (CSFs) have entered the clinical arena. Several investigators have explored, in first clinical phase I studies, different routes of administration to define the optimum biological dose, maximum tolerated dose, toxicity, and pharmacokinetics of these reagents. It has been demonstrated that recombinant human (rh) granulocyte-macrophage CSF (GM-CSF) and granulocyte CSF (G-CSF) can be safely administered over a broad dose range to increase number of circulating granulocytes in man. More recently, GM-CSF and G-CSF have been involved in phase Ib/II studies to assess the granulopoietic responses of patients with granulocytopenia due to various underlying disease states i…

Valpromide is a poor inhibitor of the cytosolic epoxide hydrolase

1989

The effect of the antiepileptics valpromide and sodium valproate on the cytosolic epoxide hydrolase was studied in human fetal liver, kidneys and adrenals and from human adult liver and kidneys. Trans-stilbene oxide was used as substrate. Valpromide (10 mM) lowered the activity of the epoxide hydrolase to one half of the control in all organs studied. Sodium valproate (10 mM) was less powerful as an inhibitor than valpromide; however, it exerted a significant inhibition in all tissues studied.

Nonperturbative treatments of nonresonant multiphoton ionization of the hydrogen atom: weak-field limit

1989

A nonperturbative treatment of the multiphoton ionization of the hydrogen atom based on the S matrix and devised for nonresonant strong-field situations is analyzed in the weak-field limit. Comparisons are presented with other S matrices as well as other nonperturbative approaches. Our treatment is found to perform generally better than similar S-matrix treatments. The usual perturbative results are recovered provided that the photon wavelengths are sufficiently short and are off resonance with the atomic transitions. Important indications are obtained as to the role of the atomic structure, the relevance of the gauge consistency, and the reliability and improvement of the present nonpertur…

Electromagnetically generated extracorporeal shock waves for gallstone lithotripsy: in vitro experiments and clinical relevance.

1989

. First generation shock wave sources have been proved to disintegrate gallstones effectively, but they require the immersion of the patient's body in a tank of water. A recently developed second generation shock wave source (Siemens-Lithostar, Erlangen, FRG) generates shock waves electromagnetically. It presents several novel features. In particular the waterbath can be omitted and due to lower shock wave pressure general anaesthesia is not required. In vitro studies showed that 36 out of 38 gallstones (11–30 mm in diameter) could be disintegrated. Two concrements resisting lithotripsy were pure white cholesterol stones. Independent of shape, size, and composition (cholesterol or pigment) …

End-plate dysfunction in acute organophosphate intoxication.

1989

Acute organophosphate intoxication resulting from suicide attempts in 14 patients produced a series of electrophysiologic abnormalities that correlated with the clinical course. Spontaneous repetitive firing of single evoked compound muscle action potentials (CMAP) was the earliest and most sensitive indicator of the acetylcholinesterase inhibition. A decrement of evoked CMAP following repetitive nerve stimulation was the most severe abnormality. At the height of the intoxication no CMAP was evoked after the first few stimuli. The decrement-increment phenomenon occurred only at milder stages of intoxication and its features are characteristic of acetylcholinesterase inhibition. These electr…

Critical behavior in quantum spin chains with composite spin

1989

Composite spin models are constructed such that, by varying two parameters, they interpolate between the spin-(1/2 antiferromagnetic Heisenberg chain and a number of spin-1 models. These include the usual Heisenberg model, the integrable spin-1 model, and the model with an exact valence-bond ground state. Finite-chain calculations are performed on the composite spin model to study its criticality, and to find if the integrable spin-1 model is a multicritical point with a finite gap generated away from it. We find indications for an extended gapless region.

Degradation of diols and formation of ethylene glycol by different yeast species

1989

Die mikrobielle Bildung von Ethylenglykol sowie der Abbau von Diolen durch Hefestamme verschiedener Gattungen wurde im synthetischen Medium und in Traubensaft untersucht. Diole (aber nicht Diethylenglykol) wurden von Arlen wieHansenula, Candida, Pichia undRhodotorula abgebaut. Von 40 uberpruften Hefen produzierten einzelne Stamme vonZygosaccharomyces bailii, Torulopsis ernobii undKluyveromyces veronae unter aeroben Bedingungen 70 bis 210 mg/l Ethylenglykol bei Zusatz von 400 mg/l Ethanolamin zum synthetischen Medium. Ohne Ethanolamin erzeugtenZ. bailii undT. ernobii im synthetischen Medium oder Traubensaft zwischen 7 bis 30 mg/l Ethylenglykol. Die Bildung von Ethylenglykol war bei aeroben K…

Orientational order of Langmuir–Blodgett films as determined by fluorescence anisotropy

1989

The orientational order parameters 〈P2〉 and 〈P4〉, of cadmium stearate Langmuir–Blodgett multilayers have been calculated from steady state fluorescence anisotropy experiments. It has been shown that it is valid to model the polarization components using the assumptions that the fluorescent probes are axially symmetric, the film is azimuthally symmetric within the plane, and that the rotational motion is slow enough to be neglected. Although the data do not preclude a dependence of anisotropy on thickness, within the sample‐to‐sample variations, there is no significant effect of thickness on orientational order. The order parameters for newly deposited films are 〈P2〉=0.33 and 〈P4〉=0.02. The …

Cytolytic T-cell clones against an autologous human melanoma: specificity study and definition of three antigens by immunoselection.

1989

Cytolytic T-lymphocyte (CTL) clones against an autologous melanoma (SK-MEL-29) were generated by mixed lymphocyte tumor culture and subsequent cloning of responder lymphocytes at limiting dilutions. These CTL clones lysed autologous melanoma but not autologous Epstein-Barr virus-transformed B cells and none of the allogeneic tumor targets included in the specificity analysis. The lysis of autologous melanoma targets could be inhibited by monoclonal antibodies against monomorphic HLA class I determinants. For proliferation of CTLs, the stimulation with the relevant target antigen on autologous tumor cells was essential. Immunoselection experiments carried out with two CTL clones revealed the…

Population processes under the influence of disasters occurring independently of population size

1989

Markov branching processes and in particular birth-and-death processes are considered under the influence of disasters that arrive independently of the present population size. For these processes we derive an integral equation involving a shifted and rescaled argument. The main emphasis, however, is on the (random) probability of extinction. Its distribution density satisfies an equation which can be solved numerically at least up to a multiplicative constant. In an example it is also found by simulation.

Feinstruktur des ventralen mandibul�ren Muskelrezeptors bei Liposcelis bostrichophilus (Insecta, Psocoptera)

1989

Der ventrale Mandibel-Muskelrezeptor wird im Kopf von Liposcelis bostrichophilus (Psocoptera) nachgewiesen. Er zieht, median der Mandibel-Gelenkachse gelegen, vom vorderen Tentorium-Arm zur hinteren Mandibel-Basis und wird also wahrend der Abduktion der Mandibel gedehnt. Der Rezeptor besteht aus einer motorisch und sensorisch innervierten Muskelfaser und 10 bipolaren, multiterminalen Sinneszellen. Einige der Sinneszellen senden ihre Dendriten ins Innere des Rezeptor-Muskels, wo sie sich verzweigen und jeweils an den Z-Scheiben enden. Die ubrigen Sinneszellen bilden mit ihren Dendriten ein Bundel, das der Oberflache des Rezeptor-Muskels anliegt. Nur die ins Innere ziehenden Dendriten weisen …

Differentiation-regulated loss of the polysialylated embryonic form and expression of the different polypeptides of the neural cell adhesion molecule…

1989

The expression of the neural cell adhesion molecule (N-CAM) on cultured murine oligodendrocytes, their precursors, and myelin was examined by indirect immunofluorescence, biosynthetic radiolabeling followed by immunoprecipitation and Western blot analysis, using antibodies specific for various forms of the molecule. In all culture systems studied, whether the oligodendrocytes were cultured as an enriched fraction containing precursor cells or in the presence of astrocytes and neurons, a similar differentiation-stage-related expression of N-CAM was seen. At early developmental stages many tetanus toxin receptor- and A2B5 antigen-positive putative oligodendrocyte precursors with bipolar morph…

Arterio-venous malformations in childhood: Clinical presentation, results after operative treatment and long-term follow-up

1989

In a series of 182 arterio-venous malformations (AVM) recently published [8] we found 33 children aged 1 to 16 years. In 28 cases, the admitting condition was hemorrhage. For 31 AVMs total excision was possible. We observed one postoperative death accounting for a mortality of 3%. All children but two were followed-up by personal examination. Two were not able to work due to severe neurological deficits and seven had not been able to get into their intended occupation.

Modulation of Nuclear Matrix-associated 2′,5′-Oligoadenylate Metabolism and Ribonuclease L Activity in H9 Cells by Human Immunodeficiency Virus

1989

Human T cells (H9), infected with the HTLV-IIIB strain of the human immunodeficiency virus (HIV-1), have been used to study the alteration of 2',5'-oligoadenylate [2'-5')A) metabolism in relation to virus production. The synthesis of (2'-5')A was determined to proceed in close association with the nuclear matrix. After HIV infection the (2'-5')A synthetase activity increased from 1.1 to 1.5 pmol of (2'-5')A synthesized/100 micrograms of nuclear matrix protein (during a 3-h in vitro incubation period) to 8.2 pmol at day 3 after infection. Then the activity dropped to the initial values. In non-infected H9 cells the (2'-5')A synthetase activity remained unchanged. Simultaneously with the decr…

Sex-specific reaction of liver ultrastructure in zebra fish (Brachydanio rerio) after prolonged sublethal exposure to 4-nitrophenol

1989

Abstract Morphological alterations of the liver of zebra fish ( Brachydanio rerio ) following prolonged exposure to 0.1, 1 and 5 mg · l −1 4-nitrophenol (4-NP) were investigated by means of light and electron microscopy. Based on marked sexual dimorphism in control animals, liver recactions were both sex- and dose-dependent. Whereas at 0.1 mg·1 −1 only minor changes could be revealed, there were numerous structural modifications at 1 mg·1 −1 : Whereas male fish primarily react with a proliferation of smooth endoplasmic reticulum, female fish display a high degree of fenetrastion within cisternate of the rough endoplasmic reticulum. Both sexes exhibit infiltration of macrophages and lymphocy…

Failure of opioids to affect excitation and contraction in isolated ventricular heart muscle

1989

The opioid agonists morphine (selective for mu-receptors) and ethylketocyclazocine (selective for kappa-receptors), at concentrations evoking strong effects in neuronal structures, did not significantly affect the configuration of the intracellularly recorded action potential and the force of contraction in ventricular heart muscle isolated from guinea pigs, rabbits and man. These results suggest that any changes of heart functions in vivo in response to opioid-like drugs are probably not mediated postsynaptically at the myocardial cell membrane but rather presynaptically, influencing the release of noradrenaline and/or acetylcholine from the nerve terminals.

Stellenwert der Skelettszintigraphie in der Diagnostik und Verlaufskontrolle des Ewing-Sarkoms

1989

The radiological and scintigraphic findings of 26 patients with histologically proven Ewing's sarcoma were analysed. Three-phase bone scan should be done early in patients presenting with pain and normal radiographs. Perfusion and metabolism of a bone lesion can be assessed by skeletal scintigraphy. Bone metastases are first seen on bone scan. In the follow-up of the patient bone scans at regular intervals are essential to detect bone metastases and tumour recurrence. The scintigraphic findings have to be correlated with radiographs and if these are negative a short-term control is indicated. Three-phase bone scans can assess the tumours response to therapy.

Amalgamation of locally finitep-groups over a countableFC-group

1989

Monte Carlo studies of anisotropic surface tension and interfacial roughening in the three-dimensional Ising model.

1989

Extensive Monte Carlo simulations of the simple cubic Ising model with nearest-neighbor ferromagnetic interactions with a tilted interface are presented for a wide range of lattice size L, temperature T, and tilt angles \ensuremath{\theta}. The anisotropic interfacial tension is studied in detail. From the small-angle data, we obtain the step free energy density ${f}_{S}$(T,L). Finite-size scaling of the step free energy density is discussed and used to probe the predicted temperature dependence of the correlation length near and above the roughening transition. The square-root temperature dependence predicted by solid-on-solid model calculations is exhibited. Finite-size scaling implies th…

Spectroscopic and chromatographic study of the interaction of nonionic micelles with the aluminum-morin complex

1989

Abstract The interaction of the aluminum-morin system with different nonionic surfactants has been studied spectrophotometrically and by high-performance liquid chromatography. It has been found that ethylene oxide-propylene oxide condensate surfactants sensitize the spectrophotometric determination of aluminum with morin more than ethylene oxide condensate surfactants. Binding ratio parameters for the different surfactants with free morin have been also estimated from the analysis of chromatographic vacant peaks.

Correlation of an estrogen receptor-related phosphoprotein with histopathological features in breast cancer.

1989

A series of 65 cases of different histological types of breast carcinoma was investigated for the immunohistochemical location of the estrogen receptor-related, 29 kD phosphoprotein using the ER-D5 monoclonal antibody.The ER-D5 response is heterogeneous in relation to some therapeutic limitations and is correlated with histopathological features of the tumors and survival. The main parameters for evaluation of breast cancers are reviewed, both those that are statistically correlated and those that are not apparently always correlated but are known to have considerable biological meaning, such as the ER-status of tumors.